An Ultra-Sensitive Aflatoxin B1 Electrochemical Immunosensor Based on a Site-Directed Antibody Conjugation Strategy

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-09-15 DOI:10.1021/acs.jafc.5c09690

Xiaotong Hao, Huang Dai, Zhengwei Zhu, Qing Yang, Min Fang, Zhiyong Gong, Xin Liu, Lin Xu

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Abstract

Aflatoxin B₁ (AFB₁) is one of the most carcinogenic and highly toxic mycotoxins, posing significant risks to human and animal health. Accurate and sensitive detection of AFB₁ is essential for ensuring food safety. In this study, we designed an ultrasensitive electrochemical immunosensor based on an AuNP-TDN-SPA-mAb immunoprobe for AFB₁ detection. This immunoprobe was developed using a site-directed antibody conjugation strategy, which enables dual modulation of antibody orientation and programmable surface distribution, thereby enhancing detection performance. Experimental results showed that the developed immunosensor exhibited a linear response range from 0.05 to 5000 pg/mL, with a limit of detection as low as 0.042 pg/mL. The immunosensor was successfully applied to the analysis of wheat and rice bran oil samples, yielding results consistent with those obtained using ultraperformance liquid chromatography (UPLC). Therefore, this site-directed antibody conjugation approach shows significant potential for broad application in the sensitive detection of mycotoxins to guarantee food safety.

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基于定点抗体偶联策略的超灵敏黄曲霉毒素B1电化学免疫传感器

黄曲霉毒素B1 （AFB1）是最具致癌性和高毒性的真菌毒素之一，对人类和动物健康构成重大风险。准确灵敏地检测AFB1对确保食品安全至关重要。在本研究中，我们设计了一种基于AuNP-TDN-SPA-mAb免疫探针的超灵敏电化学免疫传感器，用于AFB1检测。该免疫探针采用定点抗体偶联策略开发，可实现抗体取向和可编程表面分布的双重调制，从而提高检测性能。实验结果表明，该免疫传感器在0.05 ~ 5000 pg/mL范围内呈线性响应，检出限低至0.042 pg/mL。该免疫传感器成功地应用于小麦和米糠油样品的分析，所得结果与超高效液相色谱法（UPLC）一致。因此，这种定点抗体偶联方法在真菌毒素的敏感检测中具有广泛的应用潜力，可以保证食品安全。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.