Characterization of the MSI gene family in woodland strawberry Fragaria vesca and its response to cold stresses

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-09-01 DOI:10.1016/j.scienta.2025.114384

Fei Xue , Yue Zhou , Xuanyi Gong, Xinyue Gu, Xiangli Dai, Tingting Gu

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引用次数: 0

Abstract

The multicopy suppressor of IRA (MSI) is a subfamily of WD40 repeat proteins involved in histone binding, chromatin modification, and plant development. In this study, four MSI genes have been identified and characterized in the diploid woodland strawberry (Fragaria vesca) and 17 found in the octoploid cultivated strawberry (Fragaria × ananassa). Evolutionary and structural analyses revealed that MSI proteins are highly conserved across plant species and contain cis-regulatory elements responsive to hormones and stresses in their promoters. Transcriptome data showed that FvMSI genes exhibit tissue- and stage-specific expression patterns, with higher expression levels in early developmental stages and lower levels in later stages. Furthermore, the relative expression levels of FvMSI genes are modulated by cold stress. Under cold treatment, the expression levels of the four FvMSI genes increased after 8 h or seven days. Yeast two-hybrid assays demonstrated that FvMSI4/FVE (FLOWERING LOCUS VE) interacts with both FvHDA6 (HISTONE DEACETYLASE 6) and FvHOS1 (HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENES 1), interactions that resemble those observed in Arabidopsis under cold stress. This study provides a comprehensive characterization of MSI genes, which will facilitate future functional studies of MSI-involved protein complexes in strawberry development and cold stress responses.

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林地草莓草莓MSI基因家族特征及其对冷胁迫的响应

IRA的多拷贝抑制因子（MSI）是WD40重复蛋白的一个亚家族，参与组蛋白结合、染色质修饰和植物发育。本研究在二倍体森林草莓（Fragaria vesca）和八倍体栽培草莓（Fragaria × ananassa）中分别鉴定了4个MSI基因和17个MSI基因。进化和结构分析表明，MSI蛋白在植物物种中高度保守，在其启动子中含有响应激素和胁迫的顺式调控元件。转录组数据显示，FvMSI基因表现出组织和阶段特异性表达模式，在发育早期表达水平较高，在发育后期表达水平较低。此外，FvMSI基因的相对表达水平受到冷胁迫的调节。在冷处理下，4种FvMSI基因的表达量在8 h和7 d后均有所增加。酵母双杂交实验表明，FvMSI4/FVE（开花位点VE）与FvHDA6（组蛋白去乙酰化酶6）和FvHOS1（高表达渗透反应基因1）相互作用，类似于在冷胁迫下的拟南芥中观察到的相互作用。该研究提供了MSI基因的全面表征，为未来MSI蛋白复合物在草莓发育和冷胁迫反应中的功能研究提供了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.