Dietary Riboflavin Supplementation Suppresses Colorectal Cancer Progression by Restoring the Function Effector of CD8+ T Cells

IF 6.9 Q1 FOOD SCIENCE & TECHNOLOGY
Food frontiers Pub Date : 2025-07-29 DOI:10.1002/fft2.70084
Lining Chen, Yao Lu, Peng Xue, Yingying Lin, Zhen Zhang, Yuqi Wang, Yuning Zhang, Heng Quan, Yiran Wang, Huiyuan Guo
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Abstract

Tumor cells have a competitive edge in nutrient uptake, leading to immune cell nutrient deprivation within the tumor microenvironment (TME) and suppressing anti-tumor immunity. However, the key nutrient deprived by intestinal tumor cells and effective intervention strategies remains unidentified. Using organoid models and metabolomics, we clarified riboflavin as the most preferential nutrient for intestinal tumor cells over normal epithelial cells. After confirming in vitro that riboflavin supplementation did not promote tumor cell proliferation, we found in vivo that drinking water with riboflavin effectively inhibited murine colorectal tumor growth with enhanced intra-tumoral CD8+ T-cell cytotoxicity. We further demonstrated the indispensable role of adequate riboflavin in maintaining effector CD8+ T cells and revealed intestinal tumor cells overexpressed the riboflavin transporter SLC52A2, depleting riboflavin from CD8+ T cells and dampening their cytotoxicity, which can be restored by riboflavin supplementation. Our work clarified the critical nutrient and immune evasion mechanism, suggesting riboflavin supplementation as potential therapy to enhance anti-tumor immunity in colorectal cancer.

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膳食补充核黄素通过恢复CD8+ T细胞的功能效应抑制结直肠癌的进展
肿瘤细胞在营养摄取方面具有竞争优势,导致肿瘤微环境(TME)内免疫细胞营养剥夺,抑制抗肿瘤免疫。然而,肠道肿瘤细胞被剥夺的关键营养物质和有效的干预策略仍未明确。通过类器官模型和代谢组学,我们明确了核黄素是肠道肿瘤细胞比正常上皮细胞更优先的营养物质。在体外证实补充核黄素不会促进肿瘤细胞增殖后,我们在体内发现,饮用含有核黄素的水可以有效抑制小鼠结直肠癌的生长,并增强肿瘤内CD8+ t细胞的细胞毒性。我们进一步证明了充足的核黄素在维持CD8+ T细胞中不可或缺的作用,并揭示了肠道肿瘤细胞过度表达核黄素转运体SLC52A2,从而消耗CD8+ T细胞中的核黄素并抑制其细胞毒性,这可以通过补充核黄素来恢复。我们的工作阐明了关键的营养和免疫逃避机制,提示补充核黄素是增强结直肠癌抗肿瘤免疫的潜在治疗方法。
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来源期刊
CiteScore
10.50
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0.00%
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审稿时长
10 weeks
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