Oral Administration of Epigallocatechin Gallate Prevents Air Pollution-Induced Acute Lung Injury in Mice Via the Induction of Antioxidants.

IF 4.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Yui Uehara, Hitomi Mito, Ayano Shikata, Mikako Shimoda, Akio Sugimoto, Masaki Ichitani, Kenta Aso, Masahiro Kawahara, Tomonori Unno, Ken-Ichiro Tanaka
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引用次数: 0

Abstract

Health hazards caused by air pollutants are increasing worldwide (SDGs 3.9), but no established prevention methods exist. Recently, we showed that intraperitoneal administration of epigallocatechin gallate (EGCG) prevents air pollutant-induced acute lung injury. To establish EGCG ingestion as a preventive method, the efficacy of oral EGCG administration needs to be analyzed, as it is easier than that of intraperitoneal administration. Therefore, we analyzed the effects of oral EGCG administration and ad libitum consumption of an EGCG-containing diet in mice. The combination of 0.2% EGCG-containing diet and oral EGCG administration (200 mg/kg) significantly inhibited the urban aerosol (UA)-induced acute lung injury, although each treatment alone was ineffective. Specifically, compared with the UA group, the combination group showed 37.3% decrease in total cell count, 30.5% decrease in neutrophil count, 52.6% decrease in protein level, and 39.6% decrease in dsDNA level in the bronchoalveolar lavage fluid. This combination also significantly inhibited air pollutant-induced increases in proinflammatory cytokine and chemokine expression (e.g., tumor necrosis factor-α) and ROS production in mouse lungs. Moreover, this combination increased the expression of antioxidant factors, such as nuclear factor E2-related factor 2, in whole blood. Thus, this EGCG-based intervention combination limits UA-induced acute lung injury by increasing antioxidant expression. SUMMARY: The effects of a combination of EGCG-based interventions were analyzed. The combination prevented air pollutant-induced lung inflammatory responses. The combination suppressed air pollutant-induced ROS production in the lungs. The combination increased the expression of various antioxidants in whole blood.

表没食子儿茶素没食子酸酯通过诱导抗氧化剂预防空气污染引起的小鼠急性肺损伤。
在世界范围内,空气污染物造成的健康危害正在增加(可持续发展目标3.9),但没有既定的预防方法。最近,我们发现腹腔注射没食子儿茶素没食子酸酯(EGCG)可以预防空气污染物引起的急性肺损伤。为了将EGCG作为一种预防方法,需要分析口服EGCG给药的效果,因为口服EGCG比腹腔给药更容易。因此,我们分析了口服EGCG和随意食用含EGCG的饮食对小鼠的影响。含0.2% EGCG的饲粮联合口服EGCG (200 mg/kg)可显著抑制城市气溶胶(UA)诱导的急性肺损伤,但单独治疗均无效。其中,与UA组相比,联合用药组支气管肺泡灌洗液中总细胞计数下降37.3%,中性粒细胞计数下降30.5%,蛋白水平下降52.6%,dsDNA水平下降39.6%。该组合还显著抑制了空气污染物诱导的小鼠肺部促炎细胞因子和趋化因子表达(如肿瘤坏死因子-α)和ROS生成的增加。此外,该组合增加了全血中抗氧化因子,如核因子e2相关因子2的表达。因此,这种以egcg为基础的干预组合通过增加抗氧化剂表达来限制ua诱导的急性肺损伤。总结:我们分析了以卵细胞蛋白为基础的干预措施组合的效果。这种组合可以防止空气污染物引起的肺部炎症反应。这种组合抑制了空气污染物在肺部诱导的活性氧产生。该组合增加了全血中各种抗氧化剂的表达。
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来源期刊
Molecular Nutrition & Food Research
Molecular Nutrition & Food Research 工程技术-食品科技
CiteScore
8.70
自引率
1.90%
发文量
250
审稿时长
1.7 months
期刊介绍: Molecular Nutrition & Food Research is a primary research journal devoted to health, safety and all aspects of molecular nutrition such as nutritional biochemistry, nutrigenomics and metabolomics aiming to link the information arising from related disciplines: Bioactivity: Nutritional and medical effects of food constituents including bioavailability and kinetics. Immunology: Understanding the interactions of food and the immune system. Microbiology: Food spoilage, food pathogens, chemical and physical approaches of fermented foods and novel microbial processes. Chemistry: Isolation and analysis of bioactive food ingredients while considering environmental aspects.
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