Junlin Ge, , , Yuting Ye, , , Jiaxuan Yang, , , Yan Di, , , Jie Jia, , , Jie Bai, , , Xin Jia, , , Ziyan Wu, , , Xuebo Liu, , and , Xiang Duan*,
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引用次数: 0
Abstract
Dietary proteins have been demonstrated to alleviate ulcerative colitis. Phosvitin (PSV), a highly phosphorylated protein, possesses biological functions such as anti-inflammatory and antioxidant activities. This study aimed to investigate the preventive effects of PSV on dextran sulfate sodium (DSS)-induced colitis in mice and its underlying mechanisms. Following 4 weeks of PSV intervention (50 mg/kg b.w.), a colitis model was established by administering 2.5% DSS for 1 week. Results showed that PSV intervention significantly attenuated intestinal inflammation, reduced proinflammatory cytokine levels (e.g., TNF-α), and preserved intestinal barrier integrity. Furthermore, PSV modulated the gut microbiota by enhancing microbial diversity, decreasing the abundance of Escherichia-Shigella and Akkermansia, while increasing the abundance of Lachnospiraceae and Clostridium. Fecal microbiota transplantation (FMT) experiments further demonstrated the pivotal role of gut microbiota in PSV-mediated colitis prevention, as FMT from PSV-treated donor mice markedly alleviated colitis symptoms, achieving efficacy comparable to that of direct PSV intervention. These findings suggest that PSV alleviates colitis primarily through gut microbiota modulation.
期刊介绍:
The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.