Comprehensive transcriptomics analysis reveals the molecular resistance mechanisms of Guanggan (Citrus reticulata) against Phytophthora parasitica infection

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-08-01 DOI:10.1016/j.scienta.2025.114378

Yuanda LV , Bo Jiang , Yanyan Ma , Xiaomeng Li , Lina Hu , Jie Yu , Huaxue Yan

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引用次数: 0

Abstract

Phytophthora spp. are devastating oomycete pathogens cause severe diseases such as root rot and gummosis, posing a significant threat to global citrus production. Despite their economic impact, the molecular mechanisms underlying citrus-Phytophthora interactions remain poorly understood, limiting the development of resistant cultivars. Citrus reticulata var. Guanggan (GG), a semi-wild citrus genotype, has demonstrated remarkable resistance to Phytophthora infection, making it a valuable resource for disease resistance research. In this study, we performed a comparative transcriptomic analysis of the resistant GG and a susceptible citrus variety, C. sunki var. Ziyang’xiangcheng (XC), following P. parasitica infection. Our results revealed distinct genotype-specific responses, with dynamic differentially expressed genes (DEGs) enriched in key defense pathways, including plant-pathogen interaction, hormone signaling, phenylpropanoid biosynthesis, and endoplasmic reticulum (ER) protein processing. Notably, GG exhibited earlier and stronger induction of defense-related genes, such as chitinase, CaMs/CMLs, PR1 and EDS1. Functional validation via transient overexpression of eight candidate DEGs (e.g., Ciclev10029431m (PR1), Ciclev10028964m (chitinase), and phenylpropanoid biosynthesis related genes) in Nicotiana benthamiana significantly enhanced resistance to P. parasitica, confirming their role in P. parasitica defense. These findings demonstrate the GG’s multi-layered defense strategy, integrating early pathogen recognition, phytohormone signaling, secondary metabolite production, and protein homeostasis. This study identifies key genetic targets for breeding Phytophthora-resistant citrus varieties.

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综合转录组学分析揭示了广柑（Citrus reticulata）抗疫霉菌（Phytophthora parasitica）的分子机制

疫霉（Phytophthora spp.）是一种毁灭性的卵菌病原体，可引起严重的腐根病和牙龈病等疾病，对全球柑橘生产构成重大威胁。尽管它们具有经济影响，但柑橘与疫霉相互作用的分子机制仍然知之甚少，这限制了抗性品种的开发。广干柑橘（GG）是一种半野生柑橘基因型，对疫霉具有较强的抗性，是抗病研究的重要资源。在本研究中，我们对抗性GG和易感柑橘品种紫阳香城（C. sunki var. Ziyang’xiangcheng， XC）在寄生虫感染后的转录组学进行了比较分析。我们的研究结果揭示了不同的基因型特异性反应，动态差异表达基因（DEGs）在关键防御途径中富集，包括植物-病原体相互作用、激素信号、苯丙素生物合成和内质网（ER）蛋白加工。值得注意的是，GG对几丁质酶、CaMs/ cml、PR1和EDS1等防御相关基因的诱导作用更早、更强。通过短暂过表达8个候选DEGs（如：ciclev100294311m （PR1）、Ciclev10028964m（几丁质酶）和苯丙素生物合成相关基因），在本烟中进行功能验证，显著增强了对寄生蜂的抗性，证实了它们在寄生蜂防御中的作用。这些发现证明了GG的多层防御策略，整合了早期病原体识别、植物激素信号、次生代谢物产生和蛋白质稳态。本研究确定了柑橘抗蚜品种的关键遗传靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.