Characterization of the Probiotic Properties and Cholesterol Metabolism Regulation Potential of Lactiplantibacillus plantarum QL-19.

IF 4.4 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Probiotics and Antimicrobial Proteins Pub Date : 2025-08-29 DOI:10.1007/s12602-025-10724-x

Mingkai Liu, Yanlei Han, Shanshan Wang, Yiming Wang, Hui Xu

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引用次数: 0

Abstract

Lactic acid bacteria (LAB) play a crucial role in food fermentation and human health. This study introduces Lactiplantibacillus plantarum (L. plantarum) QL-19, a novel probiotic strain isolated from traditional Hunan pickles and characterized via whole-genome sequencing and in vitro functional assays. The 3,396,702 genome contains 3236 predicted coding sequences, including genes associated with stress tolerance, adhesion, and cholesterol metabolism (cbh, cfa, and hydroxymethylglutaryl-CoA synthase). Genomic analysis further revealed biosynthetic gene clusters encoding antimicrobial and antioxidant compounds, emphasizing its functional versatility. A comprehensive safety assessment confirmed that QL-19 is non-pathogenic and non-hemolytic, meeting probiotic safety standards. In HepG2 cell-based experiments, L. plantarum QL-19 reduced intracellular total cholesterol levels. RT-qPCR and Western blotting were employed to evaluate the expression of genes and proteins involved in cholesterol metabolism. The results demonstrated that QL-19 enhanced cholesterol metabolism by upregulating the expression of SREBP2, LDLR, and CYP7A1, while downregulating NPC1L1 and HMGCR. The results suggest that QL-19 facilitates cholesterol metabolism through multiple pathways. Taken together with its 75.4% in vitro cholesterol degradation rate, these findings highlight the potential of QL-19 as a cholesterol-lowering probiotic candidate for cardiovascular health applications.

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植物乳杆菌QL-19益生菌特性及胆固醇代谢调控潜力的研究

乳酸菌在食品发酵和人体健康中起着至关重要的作用。本研究从湖南传统腌菜中分离到一株新型益生菌——植物乳杆菌QL-19，并通过全基因组测序和体外功能分析对其进行了鉴定。3,396,702基因组包含3236个预测编码序列，包括与应激耐受性，粘附性和胆固醇代谢（cbh， cfa和羟甲基戊二酰辅酶a合成酶）相关的基因。基因组分析进一步揭示了抗菌和抗氧化化合物的生物合成基因簇，强调了其功能的多功能性。综合安全性评价证实，QL-19无致病性、无溶血性，符合益生菌安全标准。在基于HepG2细胞的实验中，植物L. QL-19降低了细胞内总胆固醇水平。采用RT-qPCR和Western blotting检测胆固醇代谢相关基因和蛋白的表达。结果表明，QL-19通过上调SREBP2、LDLR和CYP7A1的表达，下调NPC1L1和HMGCR的表达，从而增强胆固醇代谢。结果表明，QL-19通过多种途径促进胆固醇代谢。结合其75.4%的体外胆固醇降解率，这些发现突出了QL-19作为心血管健康应用的降胆固醇益生菌候选物的潜力。

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来源期刊

Probiotics and Antimicrobial Proteins BIOTECHNOLOGY & APPLIED MICROBIOLOGYMICROB-MICROBIOLOGY

CiteScore

11.30

自引率

6.10%

发文量

140

期刊介绍： Probiotics and Antimicrobial Proteins publishes reviews, original articles, letters and short notes and technical/methodological communications aimed at advancing fundamental knowledge and exploration of the applications of probiotics, natural antimicrobial proteins and their derivatives in biomedical, agricultural, veterinary, food, and cosmetic products. The Journal welcomes fundamental research articles and reports on applications of these microorganisms and substances, and encourages structural studies and studies that correlate the structure and functional properties of antimicrobial proteins.