Isolation and Identification of Lactic Acid Bacteria from Traditional Fermented Shredded Potato and Research on Their Prebiotic, Antioxidant, and Fermentative Properties.

IF 4.4 2区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Probiotics and Antimicrobial Proteins Pub Date : 2025-09-03 DOI:10.1007/s12602-025-10746-5

Nan Hu, Jie Ji, Hongji Zhao, Jingyi Sun, Shanzi Cong

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Abstract

The aim of this study was to screen lactic acid bacteria (LAB) suitable for starch-based fermentation from traditional fermented shredded potatoes (TFSP). Analysis of the microbial diversity by 16S rDNA sequencing in TFSP revealed that LAB were a genus with high relative abundance of the bacteria population. Thirty LAB strains were isolated and purified from TFSP. Ten LAB strains with sequence similarity greater than 98% from TFSP were obtained, and the fermentation, antioxidant, and probiotic properties were tested. The results showed that 10 strains had acid-producing abilities. Lactiplantibacillus plantarum ZYN-03, ZYN-04, ZYN-05, ZYN-09, and ZYN-10 showed strong extracellular enzyme activity and SOD enzyme activity. Lacticaseibacillus rhamnosus ZYN-08 showed a higher survival rate in 8% ethanol (v/v). Salt tolerance and nitrite degradation capacity assays revealed that Lp. plantarum ZYN-05 and ZYN-09 had high survival rates. Lp. plantarum ZYN-05 and ZYN-10 and L. rhamnosus ZYN-08 displayed good antioxidant activity, while Lp. plantarum ZYN-01 and ZYN-04 and L. rhamnosus ZYN-08 had viable counts of LAB above 5.5 log CFU/mL in gastric acid, bile, and in vitro simulated cultures. Therefore, LAB isolated from TFSP have good fermentation capacity, probiotic properties, and antioxidant activities, and have the potential application for the fermentation of plant-based raw materials.

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传统发酵马铃薯丝中乳酸菌的分离鉴定及其益生元、抗氧化和发酵特性研究

本研究旨在从传统发酵马铃薯丝（TFSP）中筛选适合淀粉发酵的乳酸菌（LAB）。通过对TFSP的16S rDNA测序分析，发现LAB是一个相对丰度较高的菌群属。从TFSP中分离纯化了30株LAB菌株。获得10株与TFSP序列相似性大于98%的LAB菌株，并对其发酵、抗氧化和益生菌性能进行了测试。结果表明，10株菌株具有产酸能力。植物乳杆菌zn -03、zn -04、zn -05、zn -09和zn -10表现出较强的胞外酶活性和SOD酶活性。鼠李糖乳杆菌zn -08在8%乙醇（v/v）中有较高的存活率。耐盐性和亚硝酸盐降解能力测定表明；植物菌zn -05和zn -09的成活率较高。Lp。植物zn -05、zn -10和鼠李糖zn -08表现出较好的抗氧化活性；植物zn -01、zn -04和鼠李糖zn -08在胃酸、胆汁和体外模拟培养中的活菌计数均在5.5 log CFU/mL以上。因此，从TFSP中分离得到的LAB具有良好的发酵能力、益生菌特性和抗氧化活性，在植物性原料发酵方面具有潜在的应用前景。

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来源期刊

Probiotics and Antimicrobial Proteins BIOTECHNOLOGY & APPLIED MICROBIOLOGYMICROB-MICROBIOLOGY

CiteScore

11.30

自引率

6.10%

发文量

140

期刊介绍： Probiotics and Antimicrobial Proteins publishes reviews, original articles, letters and short notes and technical/methodological communications aimed at advancing fundamental knowledge and exploration of the applications of probiotics, natural antimicrobial proteins and their derivatives in biomedical, agricultural, veterinary, food, and cosmetic products. The Journal welcomes fundamental research articles and reports on applications of these microorganisms and substances, and encourages structural studies and studies that correlate the structure and functional properties of antimicrobial proteins.