Bioactive Spirocyclic C-Arylglycosides From the Fungal-Fungal Co-Culture of Pestalotiopsis sp. and Nigrospora sp.

IF 2.6 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Phytochemical Analysis Pub Date : 2025-08-28 DOI:10.1002/pca.70024

Meifang Wu, Yang Jin, Ying Chen, Ziqian Zeng, Wenbin Deng, Xiangdong Su, Yongbo Xue

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引用次数: 0

Abstract

Introduction: In this study, two new papulacandin analogs (1-2), together with 12 known compounds (3-14), were identified from the co-culture of two endophytic fungi, Pestalotiopsis sp. J025 and Nigrospora sp. Wn-2-2, which were isolated from Wikstroemia nutans Champ. ex Benth.

Objectives: The aim of this study is to investigate the antifungal activity of papulacandins and other secondary metabolites from the co-culture of two endophytic fungi.

Material and methods: Their chemical structures, including relative configurations, were elucidated using extensive spectroscopic analyses, including HRESIMS, 1D and 2D NMR, ECD calculations, and quantum mechanical-NMR calculations, as well as comparison with reported analogs.

Results: Compound 3 demonstrated promising in vitro antifungal activity against fluconazole-resistant Candida albicans with a minimum inhibitory concentration in 50% isolates (MIC₅₀) of 0.57 ± 0.01 μM.

Conclusion: These findings demonstrate that the papulacandin family is a promising resource for the development of antifungal agents against invasive Candida infections.

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拟盘多毛孢和黑孢菌共培养的生物活性螺旋环c -芳基苷。

摘要本研究从Wikstroemia nutans Champ内生真菌Pestalotiopsis sp. J025和Nigrospora sp. Wn-2-2共培养中分离到2个新的papulacandin类似物（1-2）和12个已知化合物（3-14）。Benth交货。目的：研究两种内生真菌共培养的丘疹菌素及其次生代谢产物的抗真菌活性。材料和方法：它们的化学结构，包括相对构型，通过广泛的光谱分析，包括hremsims， 1D和2D NMR， ECD计算，量子力学-NMR计算，以及与报道的类似物的比较来阐明。结果：化合物3对氟康唑耐药白色念珠菌具有良好的体外抑菌活性，50%分离株的最小抑菌浓度（MIC50）为0.57±0.01 μM。结论：这些发现表明，丘疹菌素家族是开发抗侵袭性念珠菌感染药物的一个有前景的资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytochemical Analysis 生物-分析化学

CiteScore

6.00

自引率

6.10%

发文量

审稿时长

1.7 months

期刊介绍： Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.