Identification of pathogenic bacteria in saffron (Crocus sativus L.) by high-throughput sequencing and the evaluation of the antifungal properties of different plant essential oils.

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-09-02 DOI:10.1002/jsfa.70145

Zhiwen Zhang, Xinhui Weng, Tao Lv, Zhicheng Zhang, Bingbing Ma, Fujia Luan, Shaoxian Wang, Yanqi Xu, Ping Wang, Zijin Xu

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引用次数: 0

Abstract

Background: Saffron (Crocus sativus L.), a Chinese herbal medicine of significant medicinal value, is extensively cultivated worldwide. However, fungal pathogen-induced corm rot disease poses a major challenge to saffron cultivation, causing substantial economic losses and long-standing concern among researchers and industry professionals. The presemt study aimed to accurately and rapidly identify Fusarium oxysporum f. sp. crocus (FOSC) as a crocus-specific pathogen using high-throughput sequencing technology.

Results: High-throughput sequencing successfully confirmed FOSC as the pathogen responsible for crocus corm rot, with subsequent tolerance gene predictions indicating its resistance to multiple antibiotics. Among eight tested plant essential oils, Cinnamomum cassia essential oil (CC-EO) exhibited the strongest antifungal activity against FOSC. Cellular-level investigations revealed that CC-EO combats FOSC by compromising spore cell membrane integrity, inducing nuclear DNA fragmentation and promoting reactive oxygen species accumulation.

Conclusion: This study represents the first application of high-throughput sequencing to identify the crocus-specific FOSC, and demonstrates that CC-EO holds significant potential for preventing and controlling saffron corm rot through its multi-target antifungal mechanism. © 2025 Society of Chemical Industry.

查看原文本刊更多论文

藏红花（Crocus sativus L.）病原菌的高通量测序鉴定及不同植物精油抑菌性能评价。

背景：藏红花（Crocus sativus L.）是一种具有重要药用价值的中草药，在世界范围内被广泛种植。然而，真菌病原菌诱导的球茎腐病对藏红花种植构成了重大挑战，造成了巨大的经济损失，长期以来一直受到研究人员和行业专业人士的关注。本研究旨在利用高通量测序技术准确、快速地鉴定藏红花（Fusarium oxysporum f. sp. crocus， FOSC）特异性病原体。结果：高通量测序成功证实FOSC是藏红花球茎腐病的病原体，随后的耐受性基因预测表明其对多种抗生素具有耐药性。在8种植物精油中，肉桂精油（CC-EO）对FOSC的抗真菌活性最强。细胞水平的研究表明，CC-EO通过破坏孢子细胞膜完整性、诱导细胞核DNA断裂和促进活性氧积累来对抗FOSC。结论：本研究首次应用高通量测序技术鉴定藏红花特异性FOSC，表明CC-EO通过其多靶点抗真菌机制在防治藏红花球茎腐病方面具有重要潜力。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.