Effect of xylo-oligosaccharide on the in vitro proliferation of lactic acid bacteria from rabbits.

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-09-03 DOI:10.1002/jsfa.70162

Jian-Xia Yang, Hai-Ying Guo, Kexin Zhang, Yong Zuo, Yuan-Ting Zhu, Yan Zeng, Gui-Zhen Gong, Lin-Zhi Zeng, Yi-Xin Wang, Lan-Yue Chen, Yang Chen

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引用次数: 0

Abstract

Background: Rabbits often suffer from diarrhea post-weaning, necessitating eco-friendly alternatives to banned antibiotic feed additives. This study aimed to investigate the effect of xylo-oligosaccharide (XOS) on the in vitro proliferation of 94 lactic acid bacteria (LAB) strains derived from the gut of rabbits.

Results: XOS significantly enhanced the growth of 34 LAB strains (36.2%, 34/94), with Lactobacillus sp. YT155 demonstrating robust XOS metabolism. The genome of strain YT155 harbored xylABCPRT and xylCFGHT gene clusters, encoding intracellular β-xylosidase for XOS degradation. Additionally, strain YT155 exhibited tolerance to varying conditions, including temperature (37-50 °C), pH (5.0-10.0) and salinity (2-6%), and gastric and intestinal fluids. Strain YT155 also showed strong antagonistic activity against Escherichia coli and Staphylococcus aureus, and high antioxidant capacity with DPPH and hydroxyl radical scavenging rates of 100.39 ± 1.82% and 34.09% ± 1.57%, respectively. Furthermore, strain YT155 was sensitive to nine commonly used antibiotics and lacked transferable resistance genes.

Conclusion: XOS is a viable prebiotic for improving rabbit gut health by promoting beneficial LAB. Lactobacillus sp. YT155's ability to metabolize XOS and its excellent probiotic characteristics suggest it could be combined with XOS as a synbiotic to modulate gut microbiota. © 2025 Society of Chemical Industry.

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低聚木糖对家兔乳酸菌体外增殖的影响。

背景：兔子经常在断奶后腹泻，需要环保的替代品来代替禁用的抗生素饲料添加剂。本研究旨在探讨低聚木糖（XOS）对94株家兔肠道乳酸菌（LAB）体外增殖的影响。结果：XOS对34株乳酸菌生长有显著促进作用（36.2%,34/94），其中YT155乳酸菌对XOS代谢较强。菌株YT155的基因组包含xylABCPRT和xycfght基因簇，编码细胞内降解XOS的β-木糖苷酶。此外，菌株YT155对温度（37-50°C）、pH（5.0-10.0）和盐度（2-6%）以及胃液和肠液均表现出耐受性。菌株YT155对大肠杆菌和金黄色葡萄球菌具有较强的拮抗活性，对DPPH和羟基自由基的清除率分别为100.39±1.82%和34.09%±1.57%，具有较高的抗氧化能力。菌株YT155对9种常用抗生素敏感，缺乏可转移的耐药基因。结论：XOS是一种通过促进有益乳酸菌来改善家兔肠道健康的益生元。乳酸菌YT155代谢XOS的能力及其良好的益生菌特性表明，它可以与XOS联合作为合成菌来调节肠道微生物群。©2025化学工业协会。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.