Radix Actinidiae chinensis inhibits neovascularization in colorectal cancer and its mechanism.

IF 2 4区 生物学 Q3 CELL BIOLOGY
Ziqi Meng, Minyuan Chen, Jiante Li, Jieyu Li
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引用次数: 0

Abstract

Objective: Colorectal cancer is one of the most common cancers worldwide, and its angiogenesis is a key factor in tumor growth and metastasis. Radix Actinidiae chinensis is considered to have antitumor activity in traditional Chinese medicine, but its effect on neovascularization in colorectal cancer has not been clarified. Herein, we aimed to evaluate the effect of different concentrations of Radix Actinidiae chinensis on the neovascularization of colorectal cancer and explore its possible mechanisms.

Method: A mouse model of colorectal cancer was established, and mice were randomly divided into control, low-, and high-concentration groups. Then the mice in the experimental group were treated with Radix Actinidiae chinensis, and its effects on neovascularization and tumor growth were evaluated by tumor growth curve tracking, immunohistochemical analysis, vessel density assessment, RT-qPCR, and protein immunoblotting to explore the underlying mechanisms.

Results: It was shown that tumor tissues in the high-concentration group exhibited significantly slower growth in both mass and volume compared with the low-concentration and control groups. Immunohistochemical staining revealed a reduction in the expression of the vascular endothelial marker CD31 in the Radix Actinidiae chinensis treatment group. Moreover, the protein expression levels of vascular markers in tumor tissues showed a slight decrease in the low-concentration group and a marked reduction in the high-concentration group. These findings suggest that angiogenesis in the tumor microenvironment was inhibited in a concentration-dependent manner, with protein expression levels closely mirroring gene expression patterns.

Conclusion: The study found that Radix Actinidiae chinensis inhibits neovascularization in a dose-dependent manner in a mouse model of colorectal cancer. These results provide experimental support for its potential use as a therapeutic agent against colorectal cancer, suggesting that it may suppress tumor growth and metastasis by inhibiting angiogenesis.

猕猴桃抑制结直肠癌新生血管的作用及其机制。
目的:结直肠癌是世界范围内最常见的肿瘤之一,其血管生成是肿瘤生长和转移的关键因素。猕猴桃在中药中被认为具有抗肿瘤活性,但其对结直肠癌新生血管的作用尚未明确。本实验旨在评价不同浓度放线菌对结直肠癌新生血管的影响,并探讨其可能的机制。方法:建立小鼠大肠癌模型,将小鼠随机分为对照组、低浓度组、高浓度组。实验组小鼠给予猕猴桃根治,通过肿瘤生长曲线跟踪、免疫组织化学分析、血管密度评估、RT-qPCR、蛋白免疫印迹等方法评价其对新生血管和肿瘤生长的影响,探讨其作用机制。结果:高浓度组肿瘤组织在质量和体积上的生长均明显慢于低浓度组和对照组。免疫组化染色显示放线根治疗组血管内皮标志物CD31表达降低。肿瘤组织中血管标志物蛋白表达水平低浓度组略有降低,高浓度组明显降低。这些发现表明,肿瘤微环境中的血管生成以浓度依赖的方式被抑制,蛋白质表达水平与基因表达模式密切相关。结论:研究发现猕猴桃对结直肠癌小鼠模型的新生血管形成具有剂量依赖性。这些结果为其作为结直肠癌治疗剂的潜在应用提供了实验支持,表明其可能通过抑制血管生成来抑制肿瘤的生长和转移。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Histology and histopathology
Histology and histopathology 生物-病理学
CiteScore
3.90
自引率
0.00%
发文量
232
审稿时长
2 months
期刊介绍: HISTOLOGY AND HISTOPATHOLOGY is a peer-reviewed international journal, the purpose of which is to publish original and review articles in all fields of the microscopical morphology, cell biology and tissue engineering; high quality is the overall consideration. Its format is the standard international size of 21 x 27.7 cm. One volume is published every year (more than 1,300 pages, approximately 90 original works and 40 reviews). Each volume consists of 12 numbers published monthly online. The printed version of the journal includes 4 books every year; each of them compiles 3 numbers previously published online.
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