Construction of a New Drug and Agrochemical Candidates Screening Platform Utilizing Drug-Hypersensitive Fission Yeast to Discover Overlooked Natural Products

IF 3.6 2区生物学 Q2 CHEMISTRY, MEDICINAL

Journal of Natural Products Pub Date : 2025-08-23 DOI:10.1021/acs.jnatprod.5c00598

Aoi Kimishima, , , Sota Negami, , , Sota Honma, , , Shigehiro A. Kawashima, , , Yoko Yashiroda, , , Shin-ichi Fuji, , , Hiroki Kojima, , , Toshiyuki Tokiwa, , , Akihiro Sugawara, , , Yukiko Ujie, , , Akio Abe, , , Takumi Chinen, , , Minoru Yoshida, , , Takeo Usui, , and , Yukihiro Asami*,

{"title":"Construction of a New Drug and Agrochemical Candidates Screening Platform Utilizing Drug-Hypersensitive Fission Yeast to Discover Overlooked Natural Products","authors":"Aoi Kimishima, , , Sota Negami, , , Sota Honma, , , Shigehiro A. Kawashima, , , Yoko Yashiroda, , , Shin-ichi Fuji, , , Hiroki Kojima, , , Toshiyuki Tokiwa, , , Akihiro Sugawara, , , Yukiko Ujie, , , Akio Abe, , , Takumi Chinen, , , Minoru Yoshida, , , Takeo Usui, , and , Yukihiro Asami*, ","doi":"10.1021/acs.jnatprod.5c00598","DOIUrl":null,"url":null,"abstract":"<p >Natural products exhibiting selective or preferential antifungal activity against fission yeast over budding yeast might contribute greatly to new discoveries in the life sciences and new drug and agrochemical development. However, it is difficult to discover new drug and agrochemical candidates in the fission yeast screening system due to its low drug sensitivity. In this study, we constructed a new antifungal drug and agrochemical candidate screening platform using a drug-hypersensitive fission yeast strain. We executed antifungal activity profiling of our natural compound and microbial libraries against drug-hypersensitive fission yeast and multidrug-sensitive budding yeast. Ultimately, we identified MS-347a as a promising agrochemical candidate due to its excellent activity against the rice blast fungus.</p>","PeriodicalId":47,"journal":{"name":"Journal of Natural Products ","volume":"88 9","pages":"2090–2096"},"PeriodicalIF":3.6000,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Natural Products ","FirstCategoryId":"99","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acs.jnatprod.5c00598","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}

引用次数: 0

Abstract

Natural products exhibiting selective or preferential antifungal activity against fission yeast over budding yeast might contribute greatly to new discoveries in the life sciences and new drug and agrochemical development. However, it is difficult to discover new drug and agrochemical candidates in the fission yeast screening system due to its low drug sensitivity. In this study, we constructed a new antifungal drug and agrochemical candidate screening platform using a drug-hypersensitive fission yeast strain. We executed antifungal activity profiling of our natural compound and microbial libraries against drug-hypersensitive fission yeast and multidrug-sensitive budding yeast. Ultimately, we identified MS-347a as a promising agrochemical candidate due to its excellent activity against the rice blast fungus.

Abstract Image

查看原文本刊更多论文

利用药物超敏裂变酵母发现被忽视的天然产物构建新的药物和农化候选物筛选平台。

天然产物对裂变酵母比出芽酵母表现出选择性或优先的抗真菌活性，可能对生命科学、新药和农化开发的新发现作出重大贡献。然而，由于裂变酵母的药物敏感性较低，在裂变酵母筛选系统中很难发现新的药物和农化候选物。在这项研究中，我们利用一株药物超敏裂变酵母菌构建了一个新的抗真菌药物和农化候选药物筛选平台。我们对我们的天然化合物和微生物文库对药物过敏的裂变酵母和多药物敏感的芽殖酵母进行了抗真菌活性分析。最终，由于MS-347a对稻瘟病菌具有良好的活性，我们确定MS-347a是一个有前途的农化候选物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Natural Products 生物-药学

CiteScore

9.10

自引率

5.90%

发文量

294

审稿时长

2.3 months

期刊介绍： The Journal of Natural Products invites and publishes papers that make substantial and scholarly contributions to the area of natural products research. Contributions may relate to the chemistry and/or biochemistry of naturally occurring compounds or the biology of living systems from which they are obtained. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin. When new compounds are reported, manuscripts describing their biological activity are much preferred. Specifically, there may be articles that describe secondary metabolites of microorganisms, including antibiotics and mycotoxins; physiologically active compounds from terrestrial and marine plants and animals; biochemical studies, including biosynthesis and microbiological transformations; fermentation and plant tissue culture; the isolation, structure elucidation, and chemical synthesis of novel compounds from nature; and the pharmacology of compounds of natural origin.