Multiomics analysis reveals a gene regulatory network at the intersection of multiple pathways during the late stage of pear flower development

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-08-01 DOI:10.1016/j.scienta.2025.114371

Wanping Wu , Yubo Yuan , Luhan Sun , Zewen Wang , Xuxu Wang , Hao Yin , Yuanyuan Jia , Kaijie Qi , Zhihua Xie , Xu Zhang , Peng Wang , Shaoling Zhang , Xiao Wu

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引用次数: 0

Abstract

Over their evolution, plants have developed complex and precise mechanisms to regulate flowering, utilizing both internal factors within the plants themselves and external factors in the environment. Moreover, pathways such as those regulating plant stem cells, hormones, and volatile compound synthesis and metabolism are all involved in controlling flowering. However, to date, few comprehensive analyses of the core pathways involved in the flowering process have been reported. In this study, the volatile compound and hormone contents in pear blossoms at seven post-physiological differentiation stages (i.e., in the late stage of flower development) were assessed. The total volatile compound content varied greatly, ranging from 77.8 to 442.8 ng/g, and were mainly divided into seven main categories: alcohols, alkanes, ketones, terpenoids, aromatic hydrocarbons, esters, and aldehydes. The contents of the different types of compounds varied significantly. The content of gibberellin (GA) gradually decreased during pear blossom development and was significantly positively correlated with the flower volatile aldehyde and aromatic contents. Exogenous treatment with a 20 mg/L GA solution promoted flowering, whereas treatment with a 50 mg/L GA solution inhibited flowering. In addition, GA treatment changed the composition of volatile compounds, with significantly higher total and aldehyde contents than in the control group. By further integrating transcriptomics, hormonomics, and HS-SPME–GC–MS metabolomics, we identified 466 key genes involved in the pear blossom development process, including 13 core genes. This study provides profile of the pear flowering process at the gene transcript level, as well as hormone and volatile metabolism profiles for the pear flowering process, and lays a foundation for further analysis of the molecular and genetic mechanisms of pear flowering.

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多组学分析揭示了梨花发育后期多个通路交叉的基因调控网络

在植物的进化过程中，植物已经发展出复杂而精确的机制来调节开花，这既利用了植物自身的内部因素，也利用了环境中的外部因素。此外，调控植物干细胞、激素、挥发性化合物合成和代谢等途径都参与了开花的调控。然而，迄今为止，很少有报道对开花过程中涉及的核心途径进行全面分析。本研究对梨花发育后期7个生理后分化阶段（即花发育后期）的挥发性化合物和激素含量进行了测定。总挥发性化合物含量变化较大，范围为77.8 ~ 442.8 ng/g，主要分为醇类、烷烃类、酮类、萜类、芳烃类、酯类和醛类7大类。不同类型化合物的含量差异较大。赤霉素（GA）含量在梨花发育过程中逐渐降低，与花中挥发性醛和芳香含量呈极显著正相关。20 mg/L GA处理促进开花，50 mg/L GA处理抑制开花。此外，GA处理改变了挥发物的组成，总挥发物和醛含量显著高于对照组。通过进一步整合转录组学、激素组学和HS-SPME-GC-MS代谢组学，我们确定了466个参与梨花发育过程的关键基因，其中包括13个核心基因。本研究在基因转录水平上提供了梨开花过程的图谱，以及梨开花过程的激素和挥发物代谢图谱，为进一步分析梨开花的分子和遗传机制奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.