Integrated Photoelectrochemical-Fluorescence Dual-Mode Aptasensor for Sensitive Detection of Fumonisin B1

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-09-02 DOI:10.1021/acs.analchem.5c04104

Xiang Ren, Xiaoran Yang, Tingting Wu, Jingui Chen, Na Song, Dan Wu, Jinxiu Zhao, Qin Wei

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引用次数: 0

Abstract

Long-term intake of fumonisin B1 (FB1) can pose a threat to human health. It is essential to develop an accurate and sensitive method to detect FB1. In this work, a photoelectrochemical-fluorescence (PEC-FL) dual-mode aptamer sensing platform was proposed for FB1 analysis. Tb@Zr-MOF was used as a dual-functional signal label connected to p-DNA, which not only emits a fluorescent signal but also utilizes its low conductivity and steric hindrance effect to quench photocurrent and achieve signal amplification. Furthermore, BiOI/CuBi₂O₄, with a high electrode active surface area was served as a sensing matrix to provide more active sites for DNA loading. The Z-scheme heterojunction formed between BiOI and CuBi₂O₄ significantly enhanced the electron transfer rate. The sensing platform exhibited a wide linear detection range in both PEC (100 fg/mL–1 μg/mL) and FL (100 fg/mL–1 μg/mL) modes, with detection limits as low as 17.78 fg/mL (S/N = 3) and 21.93 fg/mL (S/N = 3), respectively. This work avoids environmental and operation-induced errors in single-mode detection through dual-mode cross-validation, providing a good application prospect for reliable and accurate analysis of FB1.

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集成光电化学-荧光双模适体传感器灵敏检测伏马菌素B1

长期摄入伏马菌素B1 （FB1）会对人体健康造成威胁。开发一种准确灵敏的FB1检测方法至关重要。在这项工作中，提出了一种用于FB1分析的光电化学-荧光（PEC-FL）双模式适配体传感平台。Tb@Zr-MOF作为连接p-DNA的双功能信号标签，不仅能发出荧光信号，还能利用其低电导率和位阻效应猝灭光电流，实现信号放大。此外，具有高电极活性表面积的BiOI/CuBi2O4作为传感基质，为DNA加载提供了更多的活性位点。bii和CuBi2O4之间形成的z型异质结显著提高了电子传递速率。该传感平台在PEC （100 fg/mL - 1 μg/mL）和FL （100 fg/mL - 1 μg/mL）模式下均具有较宽的线性检测范围，检出限分别低至17.78 fg/mL （S/N = 3）和21.93 fg/mL （S/N = 3）。本工作通过双模交叉验证，避免了单模检测中的环境误差和操作误差，为可靠、准确地分析FB1提供了良好的应用前景。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.