Structural elucidation of an Astragalus acidic polysaccharide and its anti-colon cancer activity through modulation of JNK/MAPK and NLRP3/IL-1β signaling pathways
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Abstract
Background
Astragalus polysaccharide, a bioactive substance with significant immunomodulatory functions, has not yet had its specific configuration–function relationship fully elucidated. In the present study, four Astragalus polysaccharide fractions with different polarities were successfully obtained through DE52 anion exchange column separation technology, and their pharmacological activities were screened.
Results
Homogeneous Astragalus polysaccharide was isolated and purified using DE52 polarity separation columns and gel column purification techniques. The types of glycosidic bonds in Astragalus polysaccharide were identified through monosaccharide composition analysis, infrared spectroscopy, molecular weight determination, and polysaccharide methylation analysis. The probable disaccharide repeating unit structure of AMPS-0.2A polysaccharide was determined to be α-(1,2)-l-Rha-α-(1,4)-d-GalA. Astragalus polysaccharide was found to induce the polarization of macrophages from the M2 phenotype to the M1 phenotype.
Conclusions
This effect was primarily mediated through the regulation of the JNK/MAPK signaling pathway and the NLRP3/IL-1β signaling pathway, thereby promoting the polarization of M1 macrophages.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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