Use of Ozonated water in the processing of human amniotic membrane and low pressure induced dehydration associated with thermal radiation

Abstract

The human amniotic membrane (HAM) is a biological material that is part of the placenta and contains compounds that enhance its applicability in the treatment of skin injuries, particularly concerning tissue regeneration. To utilize this membrane, it is essential to process it after collection, involving proper sterilization and storage. In this study, a dehydration technique for the amniotic membrane was developed using low pressure and incident thermal radiation. Histological analyses were performed after applying the sterilization protocol with ozonated water under hydrodynamic conditions, followed by submitting the samples to low pressure and light exposure. The results indicated that sterilization with ozonated water affected the integrity of the epithelium but did not induce significant changes in the morphology of the other components of the HAM. Additionally, exposure of this biomaterial to low pressure and thermal radiation was able to dehydrate it without altering the tissue integrity, which is extremely relevant considering its use in wound treatment. In this regard, the present study suggests that ozonation combined with exposure to low pressure and thermal radiation has the potential to be employed in the processing of HAM, thus favoring greater durability and ease of storage and transport of the biomaterial.