Prokaryotic Argonaute-based multiplexed detection assays for food safety: Recent advances and perspectives

IF 15.4 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Trends in Food Science & Technology Pub Date : 2025-08-19 DOI:10.1016/j.tifs.2025.105243

Letian Li , Niu Feng , Yiping Chen

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引用次数: 0

Abstract

Background

Foodborne diseases have posed serious threats to public health and resulted in substantial economic losses. To ensure food safety, it is urgent to develop rapid and sensitive multiplexed detection assays for broad-spectrum screening of foodborne hazardous factors. Prokaryotic Argonaute (pAgo), an emerging programmable endonuclease, offers precise recognition and cleavage of specific nucleic acid sequences, providing a novel analytical tool for multiplexed detection of hazardous factors. In particular, pAgo-based biosensors integrated with emerging signal transduction strategies, demonstrate enhanced sensitivity, specificity, adaptability, and reliability for multiplexed molecular diagnostics in field of food safety.

Scope and approach

This review presents a comprehensive overview of pAgo-based multiplexed detection assays for molecular diagnostics in food safety, focusing on recent advances and future potential. The principles, advantages, and key design strategies of pAgo-based multiplexed detection assays are introduced. The distinct biochemical features and detection applications of pAgos are systematically summarized, with an emphasis on developments from the past three years. Core technical challenges, including suboptimal enzymatic activity, temperature dependence, and operational complexity, are critically analyzed. Additionally, strategies involving smart nucleic acid technologies and artificial intelligence-based advanced readout systems are discussed to address limitations in sensitivity and stability of pAgo-based sensors. Impressively, the key opportunities for expanding pAgo-based multiplexed diagnostics in field of food safety are discussed.

Key findings and conclusions

As a powerful analytical toolkit, pAgos have been successfully combined with emerging signal amplification and readout strategies, opening a new way for rapid, sensitive, and multiplexed analysis of foodborne hazardous factors.

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基于argonautes的原核多重检测方法在食品安全中的应用：最新进展和展望

食源性疾病对公众健康构成严重威胁，并造成巨大的经济损失。为了确保食品安全，迫切需要开发快速、灵敏的多路检测方法，对食源性有害因素进行广谱筛选。原核Argonaute （pAgo）是一种新兴的可编程核酸内切酶，能够精确识别和切割特定的核酸序列，为多种危险因素的检测提供了一种新的分析工具。特别是，基于pago的生物传感器与新兴的信号转导策略相结合，在食品安全领域的多路分子诊断中表现出更高的灵敏度、特异性、适应性和可靠性。本文综述了基于聚乙二醇的多路检测方法在食品安全分子诊断中的应用，重点介绍了最近的进展和未来的潜力。介绍了基于聚乳酸的多路检测方法的原理、优点和关键设计策略。系统地总结了聚乙二醇砜的独特生化特性和检测应用，重点介绍了近三年来聚乙二醇砜的研究进展。核心技术挑战，包括次优酶活性，温度依赖性和操作复杂性，进行了批判性分析。此外，还讨论了涉及智能核酸技术和基于人工智能的高级读出系统的策略，以解决基于pago的传感器在灵敏度和稳定性方面的局限性。令人印象深刻的是，讨论了扩大基于pago的多路诊断在食品安全领域的关键机会。作为一个强大的分析工具，pAgos已成功地与新兴的信号放大和读出策略相结合，为快速、敏感和多路分析食源性有害因素开辟了一条新途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Trends in Food Science & Technology 工程技术-食品科技

CiteScore

32.50

自引率

2.60%

发文量

322

审稿时长

37 days

期刊介绍： Trends in Food Science & Technology is a prestigious international journal that specializes in peer-reviewed articles covering the latest advancements in technology, food science, and human nutrition. It serves as a bridge between specialized primary journals and general trade magazines, providing readable and scientifically rigorous reviews and commentaries on current research developments and their potential applications in the food industry. Unlike traditional journals, Trends in Food Science & Technology does not publish original research papers. Instead, it focuses on critical and comprehensive reviews to offer valuable insights for professionals in the field. By bringing together cutting-edge research and industry applications, this journal plays a vital role in disseminating knowledge and facilitating advancements in the food science and technology sector.