Antihypertensive effects and mechanisms of wheat oligopeptides in spontaneously hypertensive rats.

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-08-19 DOI:10.1002/jsfa.70135

Wenying Liu, Xinze Wang, Ganlu Meng, Chengjun Yang, Xinxue Zhang

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引用次数: 0

Abstract

Background: Hypertension is a significant risk factor for heart disease, stroke, and chronic kidney disease. Wheat oligopeptides (WOPs), small peptide mixtures derived from the enzymatic hydrolysis of wheat protein, contain peptides of varying molecular weights. Although previous studies have shown that plant-derived protein peptides can lower blood pressure, systematic research on WOPs and their antihypertensive mechanisms remains relatively limited. The aim of this study was to evaluate the antihypertensive efficacy of WOPs kDa and to investigate its mechanisms.

Results: The study found that WOPs demonstrated potent angiotensin-converting enzyme (ACE) inhibitory activity, achieving a 77% inhibition rate at 2.5 mg·mL^-1, and that they induced marked reductions in diastolic (DBP), systolic (SBP), and mean arterial (MBP) blood pressure in spontaneously hypertensive rats (SHRs). WOPs reduced oxidative stress-induced damage in the kidneys and myocardium of the SHR by activating the nuclear factor erythroid 2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1) signaling pathway. They also lowered serum levels of inflammatory factors in SHRs: tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-1 beta (IL-1β), endothelin-1 (ET-1), vascular endothelial growth factor A (VEGF-A), and cluster of differentiation 62E (CD62E). WOPs also alleviated kidney and myocardial tissue damage caused by hypertension through the activation of the phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) signaling pathway and regulation of angiotensin II type 1 receptor (AT1R), ACE, and endothelial nitric oxide synthase (eNOS) signaling proteins, thereby contributing to blood pressure regulation.

Conclusion: WOPs demonstrated notable antihypertensive effects in vivo. These results may provide a theoretical basis for further investigation of the in vivo antihypertensive mechanisms of WOPs and support their potential use in pharmaceutical and functional food development. © 2025 Society of Chemical Industry.

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小麦寡肽对自发性高血压大鼠的降压作用及其机制。

背景：高血压是心脏病、中风和慢性肾脏疾病的重要危险因素。小麦寡肽（WOPs）是由小麦蛋白酶解产生的小肽混合物，含有不同分子量的肽。虽然已有研究表明植物源性蛋白肽可以降低血压，但对WOPs及其降压机制的系统研究仍然相对有限。本研究的目的是评价WOPs kDa的降压效果并探讨其作用机制。结果：研究发现，WOPs对血管紧张素转换酶（ACE）有明显的抑制作用，在2.5 mg·mL-1浓度下，抑制率达到77%，并能显著降低自发性高血压大鼠（SHRs）的舒张压（DBP）、收缩压（SBP）和平均动脉压（MBP）。WOPs通过激活核因子红细胞2相关因子2 (Nrf2)/ kelch样ech相关蛋白1 （Keap1）信号通路，减少氧化应激诱导的肾和SHR心肌损伤。他们还降低了SHRs中炎症因子的血清水平：肿瘤坏死因子-α (TNF-α)、白细胞介素-6 （IL-6）、白细胞介素-1β (IL-1β)、内皮素-1 （ET-1）、血管内皮生长因子A （VEGF-A）和分化簇62E （CD62E）。WOPs还通过激活磷酸肌苷3-激酶/蛋白激酶B （PI3K/AKT）信号通路，调节血管紧张素II型1受体（AT1R）、ACE和内皮型一氧化氮合酶（eNOS）信号蛋白，减轻高血压引起的肾脏和心肌组织损伤，从而起到调节血压的作用。结论：枸杞多糖在体内具有明显的抗高血压作用。这些结果为进一步研究WOPs的体内降压机制提供了理论基础，并支持其在制药和功能食品开发中的潜在应用。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.