Crush nerve injury model in the rat sciatic nerve: A comprehensive review and validation of various methods

IF 2.3 4区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Neuroscience Methods Pub Date : 2025-08-15 DOI:10.1016/j.jneumeth.2025.110556

Daiki Kitano , Dzana Katana , Alexander R. Madanat , Anna E. Bazell , Jade M. Smith , Kacey G. Marra

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Abstract

Introduction

The rat sciatic nerve crush injury model is commonly employed to evaluate the efficacy of treatments aimed at promoting nerve regeneration. This review examines various crush techniques and the types of nerve injuries they are designed to produce.

Methods

A literature review was conducted using PubMed, Embase, Web of Science, and SCOPUS for studies published between 2010 and 2024. Studies were included if they involved surgical crush injuries on the rat sciatic nerve, performed at least one sensory functional evaluation, and compared outcomes with naive or sham controls. Crush techniques and the resulting nerve injuries were analyzed based on postoperative sensory evaluations.

Results

Of the 48 studies reviewed, 21 met the inclusion criteria. The crush techniques used included clamp (11 studies), forceps (5), clip (4), and others (1), all of which resulted in sensory impairments. Ten studies reported transient impairments with recovery to control levels, while 11 reported no recovery during the observation period (permanent). The transient group had significantly longer observation periods compared to the permanent group (57.7 vs. 17.4 days, p = 0.0009). ROC analysis determined 25 days as the optimal cutoff to distinguish transient from permanent injuries, with 90.0 % sensitivity and 81.8 % specificity.

Discussion

Regardless of the technique used, the induced nerve injury aligns with axonotmesis, characterized by spontaneous recovery over time. To accurately evaluate functional recovery, a minimum postoperative observation period of 4 weeks is recommended. This model is best suited for assessing rapid-acting agents, as spontaneous recovery may obscure the effects of slower-acting treatments.

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大鼠坐骨神经挤压神经损伤模型：各种方法的综合回顾和验证。

大鼠坐骨神经挤压损伤模型是评价促进神经再生治疗效果的常用方法。本文综述了各种压伤技术和它们所造成的神经损伤类型。方法：采用PubMed、Embase、Web of Science、SCOPUS等数据库对2010 - 2024年间发表的研究进行文献综述。如果研究涉及手术挤压大鼠坐骨神经损伤，进行至少一项感觉功能评估，并将结果与初始或假对照进行比较，则纳入研究。根据术后感觉评估，分析挤压技术和由此造成的神经损伤。结果：在回顾的48项研究中，21项符合纳入标准。使用的挤压技术包括钳（11项研究）、钳（5项研究）、夹子（4项研究）和其他技术（1项研究），所有这些技术都会导致感觉障碍。10项研究报告了恢复到控制水平的短暂性损伤，而11项研究报告在观察期间没有恢复（永久性）。与永久组相比，短暂组的观察时间明显更长（57.7天vs. 17.4天，p = 0.0009）。ROC分析确定25天是区分暂时性和永久性损伤的最佳截止时间，敏感性为90.0%，特异性为81.8%。讨论：无论使用何种技术，诱导的神经损伤与轴索痛一致，其特征是随着时间的推移自然恢复。为了准确评估功能恢复情况，建议术后至少观察4周。这个模型最适合于评估速效药物，因为自发恢复可能会掩盖慢效治疗的效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Neuroscience Methods 医学-神经科学

CiteScore

7.10

自引率

3.30%

发文量

226

审稿时长

52 days

期刊介绍： The Journal of Neuroscience Methods publishes papers that describe new methods that are specifically for neuroscience research conducted in invertebrates, vertebrates or in man. Major methodological improvements or important refinements of established neuroscience methods are also considered for publication. The Journal''s Scope includes all aspects of contemporary neuroscience research, including anatomical, behavioural, biochemical, cellular, computational, molecular, invasive and non-invasive imaging, optogenetic, and physiological research investigations.