Assessment of flow cytometric tools to characterize milk somatic cells in water buffalo.

IF 1.2 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Dairy Research Pub Date : 2025-08-18 DOI:10.1017/S0022029925000329

Maria Carmela Scatà, Francesco Grandoni, Giovanna De Matteis

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Abstract

The aim of this Research Communication was to develop new flow cytometric tools for the fine identification and characterization of milk somatic cells in water buffalo (Bubalus bubalis). Four multicolour panels of antibodies were designed to identify different subsets of live leukocytes and epithelial cells in bulk milk samples. Panel 1, including the CD18/CD172a/CD14/CD16 markers and Live/Dead vitality dye, allowed us to identify total lymphocytes, polymorphonuclear neutrophils (PMN) and monocyte/macrophage subsets. Panel 2 (CD18/CD4/CD8/δ chain/CD335 and Live/Dead dye) allowed us to identify T helper (CD4⁺), T cytotoxic (CD8⁺), γδ lymphocytes and NK cells. Panel 3 (CD18/CD79a/CD21 and Live/Dead dye) allowed us to identify total and CD21⁺ B lymphocytes. Finally, with Panel 4 (CD18/MHC-I/pan Cytokeratin and Live/Dead dye) the epithelial cells were distinguished from leukocytes. In conclusion, we propose a fine characterization of live milk somatic cell (live differential cell count (LDCC)) in buffalo species. In the future the determination of LDCC could used to identify new markers for detecting early inflammatory states of the mammary gland or for monitoring the technological properties of milks of different somatic cell composition.

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流式细胞仪对水牛乳体细胞特性的评价。

本研究的目的是开发新的流式细胞术工具，用于水牛乳体细胞的精细鉴定和表征。设计了四个多色抗体面板，以识别散装牛奶样品中不同的活白细胞和上皮细胞亚群。面板1，包括CD18/CD172a/CD14/CD16标记物和活/死活性染料，使我们能够识别总淋巴细胞，多形核中性粒细胞（PMN）和单核细胞/巨噬细胞亚群。面板2 （CD18/CD4/CD8/δ链/CD335和活/死染料）使我们能够识别T辅助细胞（CD4+）、T细胞毒性细胞（CD8+）、γδ淋巴细胞和NK细胞。面板3 （CD18/CD79a/CD21和活/死染料）使我们能够识别总淋巴细胞和CD21+ B淋巴细胞。最后，用4号图（CD18/MHC-I/pan Cytokeratin和Live/Dead dye）将上皮细胞与白细胞区分开来。总之，我们提出了水牛活乳体细胞（活差异细胞计数（LDCC））的精细表征。在未来，LDCC的测定可用于鉴定新的标志物，用于检测乳腺的早期炎症状态或监测不同体细胞组成的乳汁的技术特性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Dairy Research 农林科学-奶制品与动物科学

CiteScore

3.80

自引率

4.80%

发文量

117

审稿时长

12-24 weeks

期刊介绍： The Journal of Dairy Research is an international Journal of high-standing that publishes original scientific research on all aspects of the biology, wellbeing and technology of lactating animals and the foods they produce. The Journal’s ability to cover the entire dairy foods chain is a major strength. Cross-disciplinary research is particularly welcomed, as is comparative lactation research in different dairy and non-dairy species and research dealing with consumer health aspects of dairy products. Journal of Dairy Research: an international Journal of the lactation sciences.