Jamie K D van Wyk, Daneal C S Rorke, Johann F Gӧrgens, Eugéne van Rensburg
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引用次数: 0
Abstract
Bioaugmentation of anaerobic digestion (AD) systems is considered a cost-effective and environmentally friendly strategy to combat incomplete digestion of recalcitrant lignocellulosic substrates. This study investigated the lowest microbial inoculum size required for once-off bioaugmentation of AD cultures to enhance biomethane yield and process performance. The batch, laboratory-scale anaerobic co-digestion was carried out using pretreated corn stover (PCS) and food waste (FW), with cellulolytic Bacillus subtilis, Serratia marcescens and Bacillus licheniformis. The bioaugmentation screening was accomplished through a stepwise increase in the microbial loading using an initial standardised concentration of 0.4 × 1011 colony-forming units (CFU)/mL within the system. Bioaugmentation decreased the digestion time by up to 11 days. The inoculation of B. subtilis at a microbial concentration of 20 × 1011 CFU/mL (4.85 g DCW/L) improved the biomethane yield by 34% compared to the unaugmented control and produced 525 NmL CH4/gVS. Additionally, S. marcescens at 12 × 1011 CFU/mL doubled the volumetric methane productivity from 0.47 ± 0.02 to 1.04 ± 0.02 mL/(mL.day) when compared to the unaugmented control. The application of Nanopore sequencing after AD, to investigate the microbial community dynamics and structure in this treatment, underlined 43.52, 7.69 and 25.26% increases in the bacterial alpha diversity, namely the Shannon-, Simpson- and Observed indices, respectively. Moreover, a high abundance of between 50 and 80% of the Firmicutes population was identified.
期刊介绍：
Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes.
Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged.
The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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