Sooho Lim, Hyeonha Yoo, Giyoun Han, Woojin Kim, Minlee Kim
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引用次数: 0
Abstract
The microsporidian Vairimorpha (=Nosema) ceranae is a major pathogen affecting honeybee health worldwide, yet safe and effective control strategies remain elusive. RNA interference (RNAi) using double-stranded RNA (dsRNA) has emerged as a promising strategy for species-specific suppression with minimal environmental impact, but effective target selection and mechanistic validation remain key challenges. Here, we screened 11 dsRNAs targeting V. ceranae genes and identified three potent targets (swp25, metap2, and spp). Among them, dsRNA-swp25 exhibited the most pronounced suppression efficacy, outperforming previously validated targets. qRT-PCR analysis confirmed significant post-transcriptional gene silencing, while spore load and midgut infection area were markedly reduced in bees treated with dsRNA-swp25. Furthermore, fluorescence imaging demonstrated that Cy3-labeled dsRNA was directly internalized by V. ceranae spores in a time-dependent manner without host mediation. Collectively, these findings highlight swp25 as a high-impact RNAi target and offer mechanistic insights into pathogen-directed RNAi responsiveness. This work advances the development of practical, environmentally sustainable RNAi-based strategies for apicultural disease management.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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