Transcranial Focal Stimulation modifies genetic expression in the cerebral cortex of naive rats

IF 3.7 3区 医学 Q2 NEUROSCIENCES
Daniel Perez-Perez , Iris A. Feria-Romero , Luis Bautista-Orozco , Walter Besio , Luisa Rocha , Angelica Vega-Garcia , Omar García-Gómez , Sandra Orozco-Suarez
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Abstract

Transcranial Focal Stimulation (TFS) is an alternating-current Transcranial Electrical Stimulation technique with significant therapeutic potential. Nevertheless, the biological mechanisms responsible for the effects of TFS remain unknown. Using microarray technology, we evaluated the cerebral cortex transcriptome of rats receiving a short course (5 min) of TFS. After differential gene expression and enrichment analyses, we selected candidate genes of interest for further validation. Cerebral and hippocampal tissue of rats submitted to the same therapy were used for Western blot and immunohistochemistry to detect chosen proteins. Sham-stimulated rats were used as a reference. No differential gene expression was identified when analyzing hippocampal data. In the cerebral cortex samples, we found a total of 284 differentially expressed genes. We observed an increase in Sema4G proteins in the cerebral cortex and hippocampus (p < 0.001), and an increased expression of ZEB2 only in the hippocampus. Transcranial Focal Stimulation also increased c-Fos expression in the cerebral cortex, hippocampus, basolateral amygdala, and ventromedial hypothalamic nucleus (p < 0.001). Conclusion: A short course of TFS modifies the brain´s gene and protein expression profiles. The effects were more pronounced in the cerebral cortex than in the hippocampus. TFS also produces an increase in brain activity in cortical and subcortical regions. Additional research is necessary to validate our findings and evaluate the long-term effects of TFS.
经颅局灶性刺激改变幼稚大鼠大脑皮层的基因表达。
经颅局灶性刺激(TFS)是一种具有显著治疗潜力的交流经颅电刺激技术。然而,TFS效应的生物学机制尚不清楚。利用微阵列技术,我们评估了接受短疗程(5分钟)TFS的大鼠的大脑皮层转录组。经过差异基因表达和富集分析,我们选择了感兴趣的候选基因进行进一步验证。给予相同治疗的大鼠的大脑和海马组织使用Western blot和免疫组织化学检测选定的蛋白质。以假刺激大鼠为对照。在分析海马数据时,未发现差异基因表达。在大脑皮层样本中,我们发现了284个差异表达基因。我们观察到,Sema4G蛋白在大脑皮层和海马中表达增加(p < 0.001), ZEB2仅在海马中表达增加。经颅局灶性刺激也增加了大脑皮层、海马、基底外侧杏仁核和下丘脑腹内侧核中c-Fos的表达(p < 0.001)。结论:短时间的TFS治疗可以改变大脑的基因和蛋白表达谱。这种影响在大脑皮层中比在海马体中更为明显。TFS还会增加大脑皮层和皮层下区域的活动。需要进一步的研究来验证我们的发现并评估TFS的长期影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Brain Research Bulletin
Brain Research Bulletin 医学-神经科学
CiteScore
6.90
自引率
2.60%
发文量
253
审稿时长
67 days
期刊介绍: The Brain Research Bulletin (BRB) aims to publish novel work that advances our knowledge of molecular and cellular mechanisms that underlie neural network properties associated with behavior, cognition and other brain functions during neurodevelopment and in the adult. Although clinical research is out of the Journal''s scope, the BRB also aims to publish translation research that provides insight into biological mechanisms and processes associated with neurodegeneration mechanisms, neurological diseases and neuropsychiatric disorders. The Journal is especially interested in research using novel methodologies, such as optogenetics, multielectrode array recordings and life imaging in wild-type and genetically-modified animal models, with the goal to advance our understanding of how neurons, glia and networks function in vivo.
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