Comparative analysis on the performance of thermostability and iron incorporation of two ferritins from Sinonovacula constricta and Tegillarca granosa

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-08-05 DOI:10.1016/j.foodchem.2025.145803

Tinghong Ming, Yingwenjie Lei, Xin Luo, Chunheng Huo, Lefei Jiao, Fei Kong, Moussa Gouife, Rixin Wang, Xiurong Su, Jiajie Xu

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Abstract

It is an undisputed fact that ferritin, as a conserved and fundamental iron storage protein family, has strong tolerance to harsh environmental conditions, including heating to relatively high temperatures (>80 °C), and also displays a remarkable affinity for metal ions. However, ferritins originating from different species still retain distinct specializations. This study aims to investigate the thermostability and iron incorporation capacity of Sinonovacula constricta (ScFer) and Tegillarca granosa (TgFer). Results showed that TgFer demonstrated more increased thermostability than ScFer. Comparative biochemical analyses indicated that ScFer had a stronger not only binding interaction for Fe²⁺, but also tolerance to Fe²⁺ stress compared with TgFer. Moreover, ScFer exhibited significantly enhanced ferroxidase activity but markedly reduced iron reductive release efficiency. As a whole, these results demonstrate the high thermostability of ScFer and TgFer, and shed a promising light on their application for iron supplements.

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缢蛏和泥蚶两种铁蛋白热稳定性和铁结合性能的比较分析

铁蛋白作为一个保守的基础铁储存蛋白家族，对恶劣的环境条件具有很强的耐受性，包括加热到相对较高的温度（>80 °C），并且对金属离子也表现出显著的亲和力，这是不争的事实。然而，来自不同物种的铁蛋白仍然保留着不同的特化。本研究旨在研究缢蛏（Sinonovacula constricta, ScFer）和泥蚶（Tegillarca granosa, TgFer）的热稳定性和含铁能力。结果表明，TgFer比ScFer表现出更强的热稳定性。对比生化分析表明，与TgFer相比，ScFer不仅对Fe2+具有更强的结合作用，而且对Fe2+胁迫具有更强的耐受性。此外，ScFer显著增强了铁氧化酶活性，但显著降低了铁还原释放效率。总的来说，这些结果证明了ScFer和TgFer的高热稳定性，并为它们在铁补充剂中的应用带来了希望。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.