Extraction, purification, structure characterization of polysaccharides from Cistanche deserticola and their biological effects

IF 5.1 Q1 CHEMISTRY, APPLIED

Food Hydrocolloids for Health Pub Date : 2025-07-30 DOI:10.1016/j.fhfh.2025.100235

Faqin Tao , Baotang Zhao , Shen Song , Yunfei Xu , Ji Zhang

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Abstract

The polysaccharide fraction (CDP-D2-N1) from the stem of Cristanche deseticola was obtained after extraction and purification. The structural characteristics and in vitro antioxidant and anti-inflammatory activities of CDP-D2-N1 were investigated by physical, chemical and instrumental analyses. The results showed that CDP-D2-N1 (127.784 kDa) is a heterogeneous polysaccharide with an irregular non-crystalline structure, containing mainly Rha:Ara:Xyl:Gal:Gal:GlcA:Glc:GalA, and a molar ratio of 6.75 : 26.68 : 1.56 : 37.87 : 3.94 : 2.17 : 21.02. Combined with the results of NMR spectroscopy and methylation analyses, the main chain of CDP-D2-N1 was determined to be →4)-α-d-GalpA-(1→,→3,6)-β-d-Galp-(1→, →5)-α-l-Araf-(1→, containing a small amount of fragment →3,4)-β-d-Galp-(1→ and →3)-β-d-Galp-(1→, and the CDP-D2-N1 structure was inferred. In vitro assays demonstrated that CDP-D2-N1 possessed significant antioxidant capacity and attenuated inflammation induced by lipopolysaccharide (LPS) from Escherichia coli O55:B5 in bovine endothelial cells (bEEcs) by inhibiting the expression levels of TNF-α, IL-6, TLR 4 and IL-1.

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肉苁蓉多糖的提取、纯化、结构表征及其生物学效应

提取纯化后，得到了地衣牡丹茎多糖部分（CDP-D2-N1）。采用物理、化学和仪器分析方法研究了CDP-D2-N1的结构特征及体外抗氧化和抗炎活性。结果表明，CDP-D2-N1 （127.784 kDa）是一种不规则的非晶体结构的多相多糖，主要成分为Rha:Ara:Xyl:Gal:Gal:GlcA:Glc:GalA，摩尔比为6.75:26.68:1.56:37.87:3.94:2.17:21.02。结合核磁共振波谱和甲基化分析结果，确定了CDP-D2-N1的主链为→4)-α-d- galpa -(1→，→3,6)-β-d- galp -(1→，→5)-α-l- araf -（1→），含有少量片段→3,4)-β-d- galp -(1→和→3)-β-d- galp -（1→），推断了CDP-D2-N1的结构。体外实验表明，CDP-D2-N1通过抑制TNF-α、IL-6、tlr4和IL-1的表达，具有显著的抗氧化能力，减轻了大肠杆菌O55:B5脂多糖（LPS）在牛内皮细胞（bEEcs）中的炎症反应。

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