Dental enamel following maternal exposure to different fluoride concentrations: Physicochemical and functional approaches in rats offspring

IF 3.4 3区 医学 Q2 PHARMACOLOGY & PHARMACY
Victória Santos Chemelo , Maria Karolina Martins Ferreira , Leonardo Oliveira Bittencourt , Liliana Carolina Báez-Quintero , Juliano Pelim Pessan , Alan Rodrigo Leal Albuquerque , Rômulo Simões Angélica , Sofia Pessanha , Aline Dionizio , Marília Afonso Rabelo Buzalaf , Rafael Rodrigues Lima
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Abstract

Excessive fluoride (F) exposure is associated with adverse effects at different life stages and can affect various biological systems, including the mineralized tissues of the oral cavity. However, there is limited evidence that early F exposure during pregnancy and lactation impairs the development of offspring dental enamel. From a translational perspective, this study aimed to investigate the effects of F at different concentrations on the ultrastructural, physicochemical, and functional properties of dental enamel in the offspring of rats exposed during the prenatal and lactation periods. Pregnant Wistar rats were divided into: control (deionized water), 10 mg F/L, and 50 mg F/L. F exposure was conducted from the first day of pregnancy until the 21st day of lactation. Enamel samples from the offspring's upper incisors were collected to evaluate F levels, ultrastructural characteristics, and physicochemical composition through scanning electron microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), Raman spectroscopy, and X-ray diffraction (XRD). The results showed that increased levels of F triggered changes in phosphate and carbonate contents, with no alterations in the ultrastructure of the prisms or enamel crystallinity. Nevertheless, a significant increase in enamel hardness was observed in F exposed groups. These findings suggest that while F exposure did not affect the ultrastructure integrity of enamel, it significantly altered its chemical composition and mechanical properties. Our data suggest that maternal exposure to excessive levels of F during the prenatal and lactation periods leads to increased enamel hardness, which could impact enamel friability.
母鼠暴露于不同氟化物浓度后牙釉质:大鼠后代的理化和功能研究。
过量的氟化物(F)暴露与不同生命阶段的不利影响有关,并可影响各种生物系统,包括口腔的矿化组织。然而,有有限的证据表明,在怀孕和哺乳期早期接触F会损害后代牙釉质的发育。本研究从转译的角度,探讨不同浓度F对大鼠产前和哺乳期暴露后代牙釉质超微结构、物理化学和功能特性的影响。妊娠Wistar大鼠分为:对照组(去离子水),10 mg F/L和50 mg F/L。F暴露于妊娠第一天至哺乳期第21天。通过扫描电子显微镜(SEM)、傅里叶变换红外光谱(FTIR)、拉曼光谱(Raman Spectroscopy)和x射线衍射(XRD)等方法,采集后代上门牙牙釉质样品,评估F水平、超微结构特征和理化成分。结果表明,F水平升高会引起磷酸盐和碳酸盐含量的变化,但棱柱的超微结构和牙釉质结晶度没有变化。但氟暴露组牙釉质硬度明显升高。这些结果表明,虽然F照射不会影响牙釉质的超微结构完整性,但会显著改变牙釉质的化学成分和力学性能。我们的数据表明,母亲在产前和哺乳期暴露于过量的F会导致牙釉质硬度增加,这可能会影响牙釉质的脆弱性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.80
自引率
2.60%
发文量
309
审稿时长
32 days
期刊介绍: Toxicology and Applied Pharmacology publishes original scientific research of relevance to animals or humans pertaining to the action of chemicals, drugs, or chemically-defined natural products. Regular articles address mechanistic approaches to physiological, pharmacologic, biochemical, cellular, or molecular understanding of toxicologic/pathologic lesions and to methods used to describe these responses. Safety Science articles address outstanding state-of-the-art preclinical and human translational characterization of drug and chemical safety employing cutting-edge science. Highly significant Regulatory Safety Science articles will also be considered in this category. Papers concerned with alternatives to the use of experimental animals are encouraged. Short articles report on high impact studies of broad interest to readers of TAAP that would benefit from rapid publication. These articles should contain no more than a combined total of four figures and tables. Authors should include in their cover letter the justification for consideration of their manuscript as a short article.
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