Integrated metabolomics and transcriptomics reveal the tissue-specific distribution patterns and potential molecular mechanisms in flavonoids between Citrus reticulata ‘Shiyue Ju’ and its mutant C. reticulata ‘Denglong Ju’

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-08-01 DOI:10.1016/j.scienta.2025.114315

Qin Guan , Doudou Huang , Lan Zhang , Zongyou Lv , Wansheng Chen

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引用次数: 0

Abstract

Citrus reticulata ‘Shiyue Ju’ (STJ) and its mutant C. reticulata ‘Denglong Ju’ (DLJ) are characterized by distinct phenotypes and varying levels of nutritional components. Flavonoids, known for their significant bioactive properties in citrus, have not been systematically elucidated between these two cultivars. The present study employs comparative metabolomics to comprehensively analyze flavonoid profiles across STJ and DLJ tissues, revealing the identification of 129 flavonoid compounds predominantly represented by flavones, flavanones, and flavonols. 34 flavonoids were significantly accumulated in the peel of STJ, while another 39 flavonoids were notably enriched in the peel of DLJ. Our findings demonstrate that the distinct tissue-specific flavonoid profiles likely underpin the significant separation observed between STJ and DLJ. Transcriptomic analysis identified critical genes associated with flavonoid biosynthesis pathways: OMTs and UGTs. Notably, the intense pigmentation observed in the peel of DLJ appears to be linked to the pronounced upregulation of PAL and ANR genes. Additionally, the integration of transcriptomic and metabolomic datasets using weighted gene co-expression network analysis (WGCNA) revealed that two genes (LOC18034981 and LOC18037750) might play vital roles in the differential flavonoid production observed between STJ and DLJ. This study establishes a molecular foundation for elucidating the biosynthesis of flavonoids in these two citrus species while offering novel insights into enhancing citrus fruit quality as functional food resources.

查看原文本刊更多论文

综合代谢组学和转录组学揭示了柑桔‘石月居’与其突变体‘登龙居’黄酮类化合物的组织特异性分布规律及其潜在的分子机制

网柑‘世越柑’（STJ）及其突变体‘登龙柑’（DLJ）具有不同的表型和营养成分水平。黄酮类化合物在柑橘中具有重要的生物活性，但尚未系统地阐明这两个品种之间的关系。本研究采用比较代谢组学方法对STJ和DLJ组织的类黄酮谱进行综合分析，鉴定出以黄酮、黄酮和黄酮醇为主的129种类黄酮化合物。其中34种黄酮类化合物在STJ果皮中显著富集，另有39种黄酮类化合物在DLJ果皮中显著富集。我们的研究结果表明，不同的组织特异性类黄酮谱可能支持在STJ和DLJ之间观察到的显著分离。转录组学分析确定了与类黄酮生物合成途径相关的关键基因：OMTs和UGTs。值得注意的是，在DLJ果皮中观察到的强烈色素沉着似乎与PAL和ANR基因的明显上调有关。此外，利用加权基因共表达网络分析（WGCNA）对转录组学和代谢组学数据集进行整合，发现两个基因（LOC18034981和LOC18037750）可能在STJ和DLJ之间的类黄酮产生差异中发挥了重要作用。本研究为阐明这两种柑橘类黄酮类化合物的生物合成奠定了分子基础，同时为提高柑橘果实的功能性食品资源提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.