Roberto Márquez-Islas , Argelia Pérez-Pacheco , Marco Polo Colín-García , Yadira Béjar-Ramírez , Jesús Vargas-Sánchez , Augusto García-Valenzuela
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引用次数: 0
Abstract
Carrying out an adequate classification of elevated content of lipoproteins in the blood is of great importance in the clinical and research field. High lipoprotein levels can mask pathologies in the blood and affect the interpretation of blood results. In this study, we propose an unambiguous quantitative scale for lipemia based on optical transmittance measurements at two specific wavelengths of whole blood samples. Whole blood drawn from donors (105 in total) aged between 18 and 65 years, divided into two groups, was tested after a fasting period of 8–10 h. Trained technicians visually determined the degree of lipemia. Lipid profile analysis of the first group (48 samples) and transmittance measurements of the plasma (57 samples) of the second group were obtained. We found that the correspondence between the concentration of any of the analyzed lipoproteins and the degree of lipemia, determined visually, is not one-to-one for most of the samples. A simple theoretical analysis based on Mie scattering is presented to elucidate the possible contribution of different lipoproteins to the observed degree of lipemia in the different blood samples. Our analysis suggests that the turbidity of blood serum is due only to VLDL lipoproteins and is directly related to their size. The reclassification of the degree of lipemia using our proposed quantitative scale coincides with the original classification performed visually by the trained technician in 65 % of the cases.
期刊介绍:
Optik publishes articles on all subjects related to light and electron optics and offers a survey on the state of research and technical development within the following fields:
Optics:
-Optics design, geometrical and beam optics, wave optics-
Optical and micro-optical components, diffractive optics, devices and systems-
Photoelectric and optoelectronic devices-
Optical properties of materials, nonlinear optics, wave propagation and transmission in homogeneous and inhomogeneous materials-
Information optics, image formation and processing, holographic techniques, microscopes and spectrometer techniques, and image analysis-
Optical testing and measuring techniques-
Optical communication and computing-
Physiological optics-
As well as other related topics.