Analysis of Transdermal Distribution and Blood Components in Guinea Pigs Following the Application of Bai Jie Zi San Using Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging and Ultra-High-Performance Liquid Chromatography-Quadrupole-Time-of-Flight Mass Spectrometry.
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Abstract
Introduction: Bai Jie Zi San (BJZS) is a traditional Chinese herbal formula commonly used in acupoint sticking therapy, extensively used in clinical practice for the prevention and treatment of asthma. It has a wide application not only in China but also across various East Asian countries.
Objective: This study aimed to analyze the transdermal distribution and blood components in guinea pigs following the application of BJZS.
Methods: We used matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) to visualize the transdermal spatial distribution of BJZS components in the skin tissue of guinea pigs and ultra-high-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) to identify the components present in the blood.
Results: The results revealed significant differences in the distribution of each component across various skin areas of the guinea pigs, with the Feishu acupoint showing significantly higher concentrations compared to the other acupoints. Seventeen components were successfully identified in the blood, including eight from Corydalis Rhizoma, four from Asari Radix et Rhizoma, two from Semen Sinapis Albae, two from Rhizoma zingiberis recens, and one from Kansui Radix.
Conclusion: The study demonstrated that combining MALDI-MSI with UHPLC-Q-TOF-MS technology offered an intuitive, straightforward, and reliable approach to effectively reflect the distribution characteristics of transdermal components and identify the types of components entering the bloodstream after the application of BJZS. These findings provided valuable insights into the pharmacological mechanisms and modern development of this traditional Chinese herbal formula.
期刊介绍：
Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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