Electrical pulse generator for electroporation induction in myocytes: Compared effects on skeletal and cardiac cells

IF 2.3 4区医学 Q3 ENGINEERING, BIOMEDICAL

Medical Engineering & Physics Pub Date : 2025-07-08 DOI:10.1016/j.medengphy.2025.104393

Ahmad A. Almazloum , Paulo G. Gandra , José W.M. Bassani , Rosana A. Bassani

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引用次数: 0

Abstract

High-intensity, external electric fields (HIEF) have been used in research and therapy for abnormal generation/propagation of the cardiac electrical activity (e.g., defibrillation), and for promoting access of membrane-impermeant molecules into the cytosol through electropores. This report proposes the architecture of a pulse generator designed for both pacing and electroporation induction in isolated myocytes for the study of HIEF impact and membrane repair mechanisms. The instrument was used for quantitative characterization and comparison of the sensitivity of myocytes isolated from skeletal and cardiac muscle to HIEF-induced lethal damage, which requires severe electroporation. The pulse generator was able to produce lethal injury in both cell types, even though skeletal myocytes required fields ∼50 % greater than cardiomyocytes, thus underscoring the suitability of the proposed instrument for electroporation induction in muscle cells. It is thus likely that the reported greater myocardial vulnerability to severe electrical injury, compared to other tissues, is due to the greater cardiomyocyte sensitivity to HIEF.

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用于肌细胞电穿孔诱导的电脉冲发生器：对骨骼和心脏细胞的影响比较

高强度外电场（HIEF）已被用于心脏电活动的异常产生/传播（例如，除颤）的研究和治疗，以及促进膜外分子通过电孔进入细胞质。本报告提出了一种脉冲发生器的结构，该脉冲发生器设计用于在分离的肌细胞中起搏和电穿孔诱导，用于研究HIEF影响和膜修复机制。该仪器用于定量表征和比较从骨骼肌和心肌分离的肌细胞对hief致死性损伤的敏感性，hief致死性损伤需要严重的电穿孔。脉冲发生器能够在两种细胞类型中产生致死损伤，即使骨骼肌细胞所需的电场比心肌细胞大50%，因此强调了所提出的仪器在肌肉细胞中电穿孔诱导的适用性。因此，与其他组织相比，报告的心肌对严重电损伤的易感性更大，可能是由于心肌细胞对HIEF的敏感性更大。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Medical Engineering & Physics 工程技术-工程：生物医学

CiteScore

4.30

自引率

4.50%

发文量

172

审稿时长

3.0 months

期刊介绍： Medical Engineering & Physics provides a forum for the publication of the latest developments in biomedical engineering, and reflects the essential multidisciplinary nature of the subject. The journal publishes in-depth critical reviews, scientific papers and technical notes. Our focus encompasses the application of the basic principles of physics and engineering to the development of medical devices and technology, with the ultimate aim of producing improvements in the quality of health care.Topics covered include biomechanics, biomaterials, mechanobiology, rehabilitation engineering, biomedical signal processing and medical device development. Medical Engineering & Physics aims to keep both engineers and clinicians abreast of the latest applications of technology to health care.