Development of sensitive and specific immunochromatographic strips with nanoparticles for rapid detection of flumequine.

IF 6.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry: X Pub Date : 2025-07-09 eCollection Date: 2025-07-01 DOI:10.1016/j.fochx.2025.102771

Yuxuan Liu, Kang Jiang, Xiaoli Li, Linfang Lu, Menghan Sun, Na Li, Lakshani Madushika, Jun Yuan, Sumei Ling, Shihua Wang

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引用次数: 0

Abstract

Flumequine (Flu) is a fluoroquinolone veterinary antibiotic, which is easy to accumulate in animals, and Flu residues may cause a latent risk to human physiological health. In this study, a high-affinity monoclonal antibody (2.09 × 10⁹ M^-1) to specifically recognize Flu (anti-Flu mAb) was prepared, and then the lateral flow immunochromatographic strips (LFIS) with excellent sensitivity and specificity were developed by combining mAbs with nanoparticles. The linear detection ranges of LFIS based on gold particles (AuNP-LFIS) and gold nanoflowers (AuNF-LFIS) were 1.95-250 ng/mL and 0.39-100 ng/mL, respectively, and two types of LFIS showed high sensitivity and accuracy in actual sample detection. It is noteworthy that the AuNF-LFIS exhibited a higher sensitivity compared to that of the AuNP-LFIS. The LFIS developed in this study were considered to have great application potential in monitoring food safety because of its superiorities including simple operation, high sensitivity, prompted speed and good specificity.

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快速检测氟喹的纳米颗粒免疫层析条的研制。

氟喹（流感）是一种氟喹诺酮类兽用抗生素，易在动物体内积累，流感残留可能对人体生理健康造成潜在风险。本研究首先制备了一种特异性识别流感的高亲和力单克隆抗体（2.09 × 109 M-1）（anti-Flu mAb），然后将单克隆抗体与纳米颗粒结合，制备了具有良好灵敏度和特异性的侧流免疫层析条带（LFIS）。基于金颗粒的LFIS （AuNP-LFIS）和基于金纳米花的LFIS （AuNF-LFIS）的线性检测范围分别为1.95 ~ 250 ng/mL和0.39 ~ 100 ng/mL，两种LFIS在实际样品检测中具有较高的灵敏度和准确性。值得注意的是，与AuNP-LFIS相比，AuNF-LFIS表现出更高的灵敏度。本研究开发的LFIS具有操作简单、灵敏度高、提示速度快、特异性好等优点，在食品安全监测中具有很大的应用潜力。

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来源期刊

Food Chemistry: X CHEMISTRY, APPLIED-

CiteScore

4.90

自引率

6.60%

发文量

315

审稿时长

55 days

期刊介绍： Food Chemistry: X, one of three Open Access companion journals to Food Chemistry, follows the same aims, scope, and peer-review process. It focuses on papers advancing food and biochemistry or analytical methods, prioritizing research novelty. Manuscript evaluation considers novelty, scientific rigor, field advancement, and reader interest. Excluded are studies on food molecular sciences or disease cure/prevention. Topics include food component chemistry, bioactives, processing effects, additives, contaminants, and analytical methods. The journal welcome Analytical Papers addressing food microbiology, sensory aspects, and more, emphasizing new methods with robust validation and applicability to diverse foods or regions.