Insight into the carapace discoloration of black tiger shrimp (Penaeus monodon) during frozen storage from the perspective of protein

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-07-09 DOI:10.1016/j.foodchem.2025.145501

Chuang Pan, Jingrong Ma, Yuan Chai, Xiaoshan Long, Jianwei Cen, Chunsheng Li, Yongqiang Zhao

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Abstract

The purpose of this study was to illuminate the protein-induced shell discoloration of Penaeus monodon during frozen storage. Total of 259 differentially abundant proteins were identified. Of which, ATP-PFK and ubiquinone were screened as the significant color-related proteins by bioinformatic analysis and were verified by parallel reaction monitoring. Both proteins showed the hydrophilic properties with predominant α-helix content and belong to the crustacynin-like lipocalin protein with two active sites. Molecular dynamics simulation revealed that the interactions between both proteins and ice crystals mainly relies on hydrogen bonds and hydrophobic interactions. The ubiquinone showed a more stable and compact structure combined with ice crystals than that of ATP-PFK, but a less interaction with the surrounding water molecules. The discoloration on frozen P. monodon was closely correlated with the oxidation and denaturation of the proteins, as well as the decrease of reflection lights caused by the interaction between proteins and ice crystals.

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从蛋白质的角度研究黑虎对虾（Penaeus monodon）冷冻贮藏过程中甲壳的变色

本研究的目的是阐明单口对虾（Penaeus monodon）在冷冻贮藏过程中蛋白诱导的壳变色。共鉴定出259个差异丰度蛋白。其中，ATP-PFK和ubiquinone通过生物信息学分析筛选为重要的颜色相关蛋白，并通过平行反应监测进行验证。两种蛋白均表现出亲水性，α-螺旋含量显著，属于具有两个活性位点的类甲壳蛋白脂钙蛋白。分子动力学模拟表明，蛋白质与冰晶之间的相互作用主要依靠氢键和疏水相互作用。与ATP-PFK相比，泛醌与冰晶结合的结构更稳定、紧凑，但与周围水分子的相互作用更小。冻存单齿藻的变色与蛋白质的氧化变性以及蛋白质与冰晶相互作用引起的反射光减少密切相关。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.