{"title":"Insight into the carapace discoloration of black tiger shrimp (Penaeus monodon) during frozen storage from the perspective of protein","authors":"Chuang Pan, Jingrong Ma, Yuan Chai, Xiaoshan Long, Jianwei Cen, Chunsheng Li, Yongqiang Zhao","doi":"10.1016/j.foodchem.2025.145501","DOIUrl":null,"url":null,"abstract":"The purpose of this study was to illuminate the protein-induced shell discoloration of <em>Penaeus monodon</em> during frozen storage. Total of 259 differentially abundant proteins were identified. Of which, ATP-PFK and ubiquinone were screened as the significant color-related proteins by bioinformatic analysis and were verified by parallel reaction monitoring. Both proteins showed the hydrophilic properties with predominant α-helix content and belong to the crustacynin-like lipocalin protein with two active sites. Molecular dynamics simulation revealed that the interactions between both proteins and ice crystals mainly relies on hydrogen bonds and hydrophobic interactions. The ubiquinone showed a more stable and compact structure combined with ice crystals than that of ATP-PFK, but a less interaction with the surrounding water molecules. The discoloration on frozen <em>P. monodon</em> was closely correlated with the oxidation and denaturation of the proteins, as well as the decrease of reflection lights caused by the interaction between proteins and ice crystals.","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"6 1","pages":""},"PeriodicalIF":8.5000,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1016/j.foodchem.2025.145501","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
Abstract
The purpose of this study was to illuminate the protein-induced shell discoloration of Penaeus monodon during frozen storage. Total of 259 differentially abundant proteins were identified. Of which, ATP-PFK and ubiquinone were screened as the significant color-related proteins by bioinformatic analysis and were verified by parallel reaction monitoring. Both proteins showed the hydrophilic properties with predominant α-helix content and belong to the crustacynin-like lipocalin protein with two active sites. Molecular dynamics simulation revealed that the interactions between both proteins and ice crystals mainly relies on hydrogen bonds and hydrophobic interactions. The ubiquinone showed a more stable and compact structure combined with ice crystals than that of ATP-PFK, but a less interaction with the surrounding water molecules. The discoloration on frozen P. monodon was closely correlated with the oxidation and denaturation of the proteins, as well as the decrease of reflection lights caused by the interaction between proteins and ice crystals.
期刊介绍:
Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.