Interactions between Lipopolysaccharide and Peptide Bacteriocin BacSp222 Influence Their Biological Activities.

IF 4 2区 医学 Q2 CHEMISTRY, MEDICINAL
Justyna Śmiałek-Bartyzel, Monika Bzowska, Alicja Frączek, Iwona Wojda, Renata Mężyk-Kopeć, Piotr Bonarek, Artur Blat, Jan Rak, Paweł Mak
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Abstract

This study describes the interactions between two different pro-inflammatory factors produced by bacteria, lipopolysaccharide (LPS) from Gram-negative bacteria and the peptide BacSp222 produced by a Gram-positive zoonotic strain, Staphylococcus pseudintermedius 222. We demonstrate that the mentioned molecules interact, forming a complex, and this phenomenon selectively reduces their biological activities in vitro and in vivo. Specifically, the levels of tumor necrosis factor (TNF) and nitric oxide (NO) produced by monocyte-macrophage cells were lower in samples treated with both LPS and BacSp222 compared to those treated with LPS alone. This is most likely because BacSp222 limited the ability of LPS to stimulate the TLR4 receptor. In the Galleria mellonella larvae injected simultaneously with LPS and BacSp222, the activity of hemolymph phenoloxidase, a key component of the insect immune response, was lower than that observed in larvae injected with either LPS or BacSp222 alone. Moreover, LPS inhibited the antibacterial activity of the bacteriocin, while BacSp222 limited LPS's ability to activate a proenzyme in the Limulus amebocyte lysate test. The changes in the activities of BacSp222 and LPS were attributed to the electrostatic interactions between LPS micelles and bacteriocin molecules, resulting in a decrease in LPS aggregate size and the direct formation of a complex between them, as revealed by gel filtration and isothermal microcalorimetry.

脂多糖与肽细菌素BacSp222的相互作用影响其生物活性。
本研究描述了细菌产生的两种不同的促炎因子,革兰氏阴性细菌的脂多糖(LPS)和革兰氏阳性人畜共患病菌株假中间葡萄球菌222产生的肽BacSp222之间的相互作用。我们证明上述分子相互作用,形成复合物,这种现象选择性地降低了它们在体外和体内的生物活性。具体而言,与单独使用LPS处理的样品相比,使用LPS和BacSp222处理的样品中单核巨噬细胞产生的肿瘤坏死因子(TNF)和一氧化氮(NO)水平较低。这很可能是因为BacSp222限制了LPS刺激TLR4受体的能力。在同时注射LPS和BacSp222的mellonia幼虫中,血淋巴酚氧化酶(昆虫免疫反应的关键成分)的活性低于单独注射LPS或BacSp222的幼虫。此外,LPS抑制了细菌素的抗菌活性,而BacSp222在鲎细胞裂解液试验中限制了LPS激活一种前酶的能力。凝胶过滤和等温微量热分析表明,BacSp222和LPS活性的变化是由于LPS胶束与细菌素分子之间的静电相互作用,导致LPS聚集体尺寸减小,并在两者之间直接形成复合物。
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来源期刊
ACS Infectious Diseases
ACS Infectious Diseases CHEMISTRY, MEDICINALINFECTIOUS DISEASES&nb-INFECTIOUS DISEASES
CiteScore
9.70
自引率
3.80%
发文量
213
期刊介绍: ACS Infectious Diseases will be the first journal to highlight chemistry and its role in this multidisciplinary and collaborative research area. The journal will cover a diverse array of topics including, but not limited to: * Discovery and development of new antimicrobial agents — identified through target- or phenotypic-based approaches as well as compounds that induce synergy with antimicrobials. * Characterization and validation of drug target or pathways — use of single target and genome-wide knockdown and knockouts, biochemical studies, structural biology, new technologies to facilitate characterization and prioritization of potential drug targets. * Mechanism of drug resistance — fundamental research that advances our understanding of resistance; strategies to prevent resistance. * Mechanisms of action — use of genetic, metabolomic, and activity- and affinity-based protein profiling to elucidate the mechanism of action of clinical and experimental antimicrobial agents. * Host-pathogen interactions — tools for studying host-pathogen interactions, cellular biochemistry of hosts and pathogens, and molecular interactions of pathogens with host microbiota. * Small molecule vaccine adjuvants for infectious disease. * Viral and bacterial biochemistry and molecular biology.
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