Effect of photobiomodulation on rat parotid gland cells after ionizing radiation – an experimental study in animal model – Part 1

IF 2 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Stomatology Oral and Maxillofacial Surgery Pub Date : 2025-10-01 DOI:10.1016/j.jormas.2025.102485

Cristina Paixão Durães , Larissa Lopes Fonseca , Sérgio Henrique Sousa Santos , Arlen de Paulo Santiago Filho , Marise Fagundes Silveira , Lorena dos Reis Pereira Queiroz , Allysson Steve Mota Lacerda , Alfredo Maurício Batista de Paula , Lucyana Conceição Farias , André Luiz Sena Guimarães

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引用次数: 0

Abstract

Background

Radiotherapy for head and neck cancers often impairs salivary gland function. Photobiomodulation (PBM) has emerged as a potential intervention to mitigate radiation-induced tissue damage. This study aimed to evaluate the effects of PBM on the parotid glands of Wistar rats subjected to ionizing radiation.

Methods

Forty-two male Wistar rats (7–8 weeks old) were randomly assigned to seven groups (n = 6). G1: control; G2: radiotherapy only (20 Gy); G3: PBM only; G4–G7: PBM combined with radiotherapy. PBM was applied using the Laser Duo (MMOptics®) with 660 nm and/or 808 nm wavelengths, varying in dose and number of application points. The histological analysis focused on preserving the acinar area. Receiver operating characteristic (ROC) curve analysis was used to determine the threshold for preserved acinar architecture.

Results

The group receiving combined PBM (660 nm + 808 nm, 54 J per gland; G5) demonstrated the highest preservation of acinar morphology, closely resembling the control. Groups receiving isolated red or infrared wavelengths (G4, G6) showed moderate protection. The lowest dose group (G7, 1.8 J) was ineffective.

Conclusion

PBM significantly reduced RT-induced damage to the parotid glands in a dose-dependent manner. The red laser protocol at 27 J per gland showed the highest level of tissue preservation. These findings support the potential of PBM as a protective adjunct during radiation therapy (RT) and justify further studies to standardize optimal clinical parameters.

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电离辐射后光生物调节对大鼠腮腺细胞的影响-动物模型的实验研究-第一部分。

背景：头颈部肿瘤的放疗常损害唾液腺功能。光生物调节（PBM）已成为减轻辐射引起的组织损伤的潜在干预措施。本研究旨在评价PBM对电离辐射Wistar大鼠腮腺的影响。方法：选取7 ~ 8周龄雄性Wistar大鼠42只，随机分为7组（n = 6）。G1:控制;G2：仅放疗（20 Gy）；G3：仅PBM；G4-G7: PBM联合放疗。PBM应用于激光Duo （MMOptics®），波长为660 nm和/或808 nm，剂量和应用点数量不同。组织学分析的重点是保留腺泡区域。采用受试者工作特征（ROC）曲线分析确定保留腺泡结构的阈值。结果：联合PBM组(660 nm + 808 nm， 54 J /腺体；G5)表现出最高的腺泡形态保存，与对照组非常相似。接收孤立红色或红外波长（G4, G6）的组表现出中等程度的保护。最低剂量组（G7, 1.8 J）无效。结论：PBM显著降低rt诱导的腮腺损伤呈剂量依赖性。每个腺体27 J的红色激光方案显示最高水平的组织保存。这些发现支持了PBM作为放射治疗（RT）期间的保护辅助的潜力，并证明了进一步研究标准化最佳临床参数的合理性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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