Xanthomonas campestris Employs the RND Family Pump HepABCD for Phenolic Acid Efflux and Enhancing Viability and Antioxidant Activity.

IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Kai Song, Ying Cui, Lin Li, Jia-Yuan Wang, Si-Nan Li, Yu-Cheng Gu, Ya-Wen He, Lian Zhou
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引用次数: 0

Abstract

Xanthomonas campestris pv. campestris (Xcc) is the causal pathogen of black rot in cruciferous plants. Upon infection, Xcc triggers the accumulation of some phenolic acids in the host plants. The mechanism by which Xcc copes with these defense compounds remains unclear. Here, we revealed that Xcc exports benzoic acid, cinnamic acid, and their monohydroxylated derivatives through the resistance-nodulation-division family efflux pump HepABCD. This efflux system influenced glutathione and catalase levels in the wild-type strain XC1, affecting cell viability and modulating ROS levels. We identified HepR as a sensor for 4-hydroxybenzoic acid (4-HBA). The tryptophan residue W22 is critical for HepR binding to 4-HBA. Binding of 4-HBA caused HepR to dissociate from its promoter Phep and induced hepABCD expression. Additionally, HepR acts as a redox sensor, and cysteine-to-serine mutations at C39 or C77 significantly reduced its binding affinity to Phep. Collectively, these findings highlight the crucial role of phenolic acid efflux in Xcc viability and host colonization.

油菜黄单胞菌利用RND家族泵HepABCD进行酚酸外排,提高生存能力和抗氧化活性。
油菜黄单胞菌。campestris (Xcc)是引起十字花科植物黑腐病的病原菌。感染后,Xcc会在寄主植物中引发一些酚酸的积累。Xcc应对这些防御化合物的机制尚不清楚。在这里,我们发现Xcc通过抗性-结节分裂家族外排泵HepABCD输出苯甲酸、肉桂酸及其单羟基化衍生物。这种外排系统影响野生型菌株XC1的谷胱甘肽和过氧化氢酶水平,影响细胞活力并调节ROS水平。我们确定HepR是4-羟基苯甲酸(4-HBA)的传感器。色氨酸残基W22是HepR与4-HBA结合的关键。4-HBA的结合导致HepR与其启动子Phep分离并诱导hepABCD表达。此外,HepR作为氧化还原传感器,C39或C77处的半胱氨酸-丝氨酸突变显著降低了其与Phep的结合亲和力。总的来说,这些发现强调了酚酸外排在Xcc活力和宿主定植中的关键作用。
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来源期刊
Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry 农林科学-农业综合
CiteScore
9.90
自引率
8.20%
发文量
1375
审稿时长
2.3 months
期刊介绍: The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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