Immobilization of Chenopodium murale-Derived Phytase on Novel Carriers: A Sustainable Approach to Enhance Nutrient Bioavailability in Cereal-Based Foods.

IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Khalil Ur Rehman, Ehab A Abdelrahman, Mohammed Alissa, Abdullah Alghamdi, Suad A Alghamdi, Mohammed A Alshehri, Ghfren S Aloraini, Abdullah Albelasi, Mortaga M Abou-Krisha, Abdulrahman G Alhamzani
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引用次数: 0

Abstract

This study investigates the immobilization of phytase onto three solid carriers─sodium alginate/poly(vinyl alcohol) (AlgNa/PVA) beads, glass microspheres, and cellulose beads─for the purpose of phytic acid hydrolysis in food matrices. Phytase was either entrapped within the matrix (AlgNa/PVA, cellulose) or covalently bonded (glass microspheres) for enhanced stability and reusability. Glass microspheres demonstrated superior catalytic performance with higher activity retention and reusability and were thus selected for optimization. The hydrolytic activity, evaluated using p-nitrophenyl phosphate (p-NPP) at pH 5.5 and 50 °C, resulted in a rate constant (K) of 0.024 min-1, with a half-hydrolysis time (τ50) of 36.1 min and full hydrolysis (τ_complete) achieved within 110 min. The activity of the free enzyme was strongly inhibited by Cu2-, Hg2-, Fe2-, Mg2-, Zn2-, and Ca2-, while the immobilized enzyme showed resilience, with only Mg2- causing notable inhibition. The hydrolytic efficiencies of both free and immobilized phytases were evaluated in different legumes and cereals, including broad beans, chickpeas, peanuts, peas, pinto beans, brass, maize, dry corn, oats, rye, wheat, green lentils, and red lentils, showing a significant reduction in phytic acid content and confirming the enzyme's broad substrate range and practical application potential in food processing.

在新型载体上固定化藜草衍生植酸酶:提高谷类食品营养生物利用度的可持续途径。
本研究探讨了植酸酶在海藻酸钠/聚乙烯醇(AlgNa/PVA)微球、玻璃微球和纤维素微球三种固体载体上的固定化,以水解食物基质中的植酸。植酸酶被包裹在基质(海藻/聚乙烯醇,纤维素)或共价结合(玻璃微球)中,以提高稳定性和可重复使用性。玻璃微球表现出优异的催化性能,具有较高的活性保持率和可重复使用性,因此选择进行优化。对硝基苯基磷酸(p-NPP)在pH 5.5和50°C条件下的水解活性测定结果表明,水解速率常数(K)为0.024 min-1,半水解时间(τ50)为36.1 min,全水解时间(τ_complete)在110 min内完成。游离酶的活性受到Cu2-、Hg2-、Fe2-、Mg2-、Zn2-和Ca2-的强烈抑制,而固定化酶表现出弹性,只有Mg2-具有明显的抑制作用。在不同的豆类和谷物中,包括蚕豆、鹰嘴豆、花生、豌豆、扁豆、铜、玉米、干玉米、燕麦、黑麦、小麦、绿扁豆和红扁豆,对游离和固定化植酸酶的水解效率进行了评估,结果表明植酸含量显著降低,证实了该酶广泛的底物范围和在食品加工中的实际应用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry 农林科学-农业综合
CiteScore
9.90
自引率
8.20%
发文量
1375
审稿时长
2.3 months
期刊介绍: The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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