Yang Li, Yixuan Bian, Regan McMath, James Davis, Pagona Papakonstantinou, Yuanhua Shao, Meixian Li
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引用次数: 0
Abstract
Chronic kidney disease (CKD) is a global public health issue, with high prevalence and mortality. As cystatin C is a biomarker for the early diagnosis of CKD, there is an urgent need to develop a simple and inexpensive method for the detection of Cys C in human plasma. Herein, a sensitive method with flexible electrochemical aptamer-based (E-AB) sensing interfaces has been established using poly(3,4-ethylenedioxythiophene)-poly(styrenesulfonate)-treated paper-based electrodes. Introduction of MoS2 quantum dots increased the specific surface area and realized effective immobilization of the thiolated Cys C aptamer (Cys C Apt) modified with methylene blue through the formation of disulfide bonds. The E-AB sensing interfaces were successfully applied to the quantification of Cys C in human venous whole blood and plasma samples based on the binding-induced conformational change mechanism of Cys C Apt by employing square wave voltammetry. The flexible E-AB sensing interfaces show significant potential to be developed into wearable patches for in vivo detection of Cys C, which will pave the way for early diagnosis and long-term monitoring of CKD.
慢性肾脏疾病(CKD)是一个全球性的公共卫生问题,具有很高的患病率和死亡率。由于胱抑素C是CKD早期诊断的生物标志物,因此迫切需要开发一种简单、廉价的检测人血浆中胱抑素C的方法。本文采用聚(3,4-乙烯二氧噻吩)-聚苯乙烯磺酸盐处理的纸基电极,建立了一种柔性电化学适配体(E-AB)传感界面的灵敏方法。引入二硫化钼量子点增加了比表面积,并通过形成二硫键实现了亚甲基蓝修饰的硫代Cys C适配体(Cys C Apt)的有效固定化。基于Cys C - Apt结合诱导构象变化机制,采用方波伏安法,将E-AB传感接口成功应用于人体静脉全血和血浆样品中Cys C的定量。灵活的E-AB传感接口显示出开发可穿戴贴片用于体内检测Cys - C的巨大潜力,这将为CKD的早期诊断和长期监测铺平道路。
期刊介绍:
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.