Effects of 2,4-Di‑tert-butylphenol treatment on the active constituents of Lilium brownii var. viridulum and its mechanism

IF 4.2 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-07-01 DOI:10.1016/j.scienta.2025.114251

Xin Yu , Jiani Li , Xingjie Li , Xiaolong Liu , Qingning Deng , Qi Huang , Qineng Lu , Rungen Li

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Abstract

Continuous cropping obstacles in Lilium brownii var. viridulum (L. brownii), a medicinal plant, are significantly influenced by autotoxicity, yet its impact on active components remains unexplored. This study investigates the effects of varying concentrations (0.02–2 mg mL^-1) of the autotoxin 2,4-di‑tert-butylphenol (2,4-DTBP) on the active constituents of L. brownii bulbs across three developmental stages: initial bulb formation (May 7, Stage 1), mid-bulb formation (June 16, Stage 2), and maturity (July 21, Stage 3). High 2,4-DTBP concentrations (2 mg mL^-1) enhanced reactive oxygen species (ROS) scavenging and increased total phenols, flavonoids, and saponins. Transcriptomic analysis revealed that 2,4-DTBP induced significant changes in gene expression in the phenylpropanoid and flavonoid biosynthesis pathways, with 76 and 26 DEGs in Stage 2, respectively, decreasing to 48 and 6 DEGs in Stage 3. Metabolomic profiling showed significant upregulation of phenolic and flavonoid compounds in Stage 2, with notable accumulation of dihydrocaffeic acid 3-O-glucuronide and typhaneoside. Correlation analysis identified 80 genes strongly associated with flavonoid metabolites. These findings elucidate the mechanisms underlying changes in L. brownii’s active components under autotoxic stress, providing theoretical support for optimizing its cultivation and medicinal applications.

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2,4-二叔丁基苯酚处理对百合有效成分的影响及其机制

药用植物百合（Lilium brownii var. viridulum， L. brownii）连作障碍受到自毒性的显著影响，但其对活性成分的影响尚不清楚。本研究研究了不同浓度（0.02-2 mg mL-1）的自毒素2,4-二叔丁基酚（2,4- dtbp）对L. brownii球茎在三个发育阶段的活性成分的影响：球茎初始形成（5月7日，第1阶段），球茎中期形成（6月16日，第2阶段）和成熟（7月21日，第3阶段）。高2,4- dtbp浓度（2 mg mL-1）增强活性氧（ROS）清除和增加总酚，类黄酮和皂苷。转录组学分析显示，2,4- dtbp诱导苯丙素和类黄酮生物合成途径的基因表达发生了显著变化，第2阶段分别为76和26个deg，第3阶段分别为48和6个deg。代谢组学分析显示，在第2阶段，酚类和类黄酮化合物显著上调，二氢咖啡酸3- o -葡萄糖醛酸和伤寒皂苷显著积累。相关分析鉴定出80个与类黄酮代谢产物密切相关的基因。这些研究结果阐明了褐乳杆菌在自毒胁迫下活性成分变化的机制，为其优化栽培和药用提供了理论支持。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.