Dual-Channel Catalytic Immunochromatography Empowered by Machine Learning: Ultrasensitive Detection of Escherichia coli O157:H7 via Magnetic CoFe2O4@HRP Nanocomposites.
{"title":"Dual-Channel Catalytic Immunochromatography Empowered by Machine Learning: Ultrasensitive Detection of Escherichia coli O157:H7 via Magnetic CoFe2O4@HRP Nanocomposites.","authors":"Huiqi Yan,Ying Wang,Yuting Zhuang,Yuanyuan Cao,Boyang Sun,Qinlin Feng,Haiyu Wu,Jinbo Cao,Chenyu Xuan,Zeyu Lu,Kaixuan Ma,Le Zhou,Li Wang","doi":"10.1021/acs.analchem.5c02395","DOIUrl":null,"url":null,"abstract":"Traditional immunochromatographic test strips face significant limitations in detecting trace levels of Escherichia coli O157:H7 due to insufficient sensitivity and reliability. To address this challenge, we developed a novel \"three-In-One\" nanoplatform based on magnetic CoFe2O4 NPs functionalized with horseradish peroxidase (HRP) for dual-channel lateral flow immunoassay (LFIA). The secondary catalytic channel, leveraging HRP-mediated oxidation of 3,3',5,5'-tetramethylbenzidine (TMB), enables signal amplification, achieving an unprecedented detection limit of 9 CFU/mL─a 100-fold improvement over conventional gold nanoparticle-based LFIA (930 CFU/mL) and a 10-fold enhancement compared to the noncatalyzed CoFe2O4 system (93 CFU/mL). The CoFe2O4@HRP nanocomposite demonstrates remarkable synergistic effects, combining the magnetic separation capability of CoFe2O4 with the catalytic activity of HRP. This integration not only enhances detection sensitivity but also improves the aqueous stability and antibody loading capacity. In real food sample analyses (pork and milk), the system exhibits excellent accuracy (recovery rate: 89.29-110.71%) and precision (RSD: 3.31-7.93%). To further optimize detection performance, we implemented a robust machine learning framework incorporating deep neural networks (DNN), random forest regression, and k-nearest neighbors algorithms. This predictive model achieved exceptional agreement with experimental results (R2 > 0.999), 100% classification accuracy at the order-of-magnitude level, and >95% of predictions within Bland-Altman agreement limits. This work establishes a new paradigm for foodborne pathogen detection by synergistically combining nanomaterial engineering with artificial intelligence, offering a novel paradigm in rapid, ultrasensitive, and quantitative diagnostics for food safety monitoring and clinical applications.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"76 1","pages":""},"PeriodicalIF":6.7000,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.analchem.5c02395","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Traditional immunochromatographic test strips face significant limitations in detecting trace levels of Escherichia coli O157:H7 due to insufficient sensitivity and reliability. To address this challenge, we developed a novel "three-In-One" nanoplatform based on magnetic CoFe2O4 NPs functionalized with horseradish peroxidase (HRP) for dual-channel lateral flow immunoassay (LFIA). The secondary catalytic channel, leveraging HRP-mediated oxidation of 3,3',5,5'-tetramethylbenzidine (TMB), enables signal amplification, achieving an unprecedented detection limit of 9 CFU/mL─a 100-fold improvement over conventional gold nanoparticle-based LFIA (930 CFU/mL) and a 10-fold enhancement compared to the noncatalyzed CoFe2O4 system (93 CFU/mL). The CoFe2O4@HRP nanocomposite demonstrates remarkable synergistic effects, combining the magnetic separation capability of CoFe2O4 with the catalytic activity of HRP. This integration not only enhances detection sensitivity but also improves the aqueous stability and antibody loading capacity. In real food sample analyses (pork and milk), the system exhibits excellent accuracy (recovery rate: 89.29-110.71%) and precision (RSD: 3.31-7.93%). To further optimize detection performance, we implemented a robust machine learning framework incorporating deep neural networks (DNN), random forest regression, and k-nearest neighbors algorithms. This predictive model achieved exceptional agreement with experimental results (R2 > 0.999), 100% classification accuracy at the order-of-magnitude level, and >95% of predictions within Bland-Altman agreement limits. This work establishes a new paradigm for foodborne pathogen detection by synergistically combining nanomaterial engineering with artificial intelligence, offering a novel paradigm in rapid, ultrasensitive, and quantitative diagnostics for food safety monitoring and clinical applications.
期刊介绍:
Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.