The Functional Ability of Stingless Bees (Lepidotrigona) Propolis on Inflammation-Related Disorders.

IF 4.2 2区 农林科学 Q1 FOOD SCIENCE & TECHNOLOGY
Supawan Sunthorn, Yong-Chao Su, Chun-Hui Chiu, Yung-Lin Chu
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引用次数: 0

Abstract

The large majority of the bees in the Apidae family are honey bees and stingless bees. Despite honey bees (Apis mellifera) being widely distributed worldwide, Lepidotrigona sp. is a particular genuine stingless bee that can be found only in the Southeast Asia region and Taiwan. Hence, the objectives of this study were firstly to identify Lepidotrigona propolis as an alternative medicine and investigate the anti-inflammatory effects of on lipopolysaccharide (LPS) induced RAW 264.7 macrophage cells by the determination of cell viability, dendritic cell morphology, nitric oxide (NO) production, reactive oxygen species (ROS) detection, and inflammation-related proteins analysis. In this study, the polyphenols and flavones in the propolis have been detected by UPLC-ESI-MS/MS. Compared to the LPS group, this propolis type also showed decreased NO and ROS production and a change in cell morphology. Additionally, Lepidotrigona propolis can downregulate the expression of some inflammation-related proteins, such as iNOS, TNF-α, NF-κB, and IL-6. In summary, our study has unveiled the diverse therapeutic properties of Lepidotrigona propolis, particularly its potent anti-inflammatory effects. This discovery opens up a promising avenue for developing a novel therapeutic alternative for human health, instilling hope for the future of medical science.

无刺蜂(Lepidotrigona)蜂胶对炎症相关疾病的功能能力
蜜蜂科的大多数蜜蜂是蜜蜂和无刺蜜蜂。尽管蜜蜂(Apis mellifera)广泛分布在世界各地,但Lepidotrigona sp.是一种特殊的真正的无刺蜜蜂,只能在东南亚地区和台湾找到。因此,本研究首先通过检测脂多糖(LPS)诱导的巨噬细胞活力、树突状细胞形态、一氧化氮(NO)生成、活性氧(ROS)检测和炎症相关蛋白分析,确定Lepidotrigona蜂胶作为一种替代药物,并研究其对RAW 264.7巨噬细胞的抗炎作用。本研究采用UPLC-ESI-MS/MS对蜂胶中的多酚类和黄酮类进行了检测。与LPS组相比,该蜂胶类型也显示出NO和ROS的产生减少以及细胞形态的变化。此外,Lepidotrigona蜂胶可以下调一些炎症相关蛋白的表达,如iNOS、TNF-α、NF-κB和IL-6。总之,我们的研究揭示了枸杞蜂胶的多种治疗特性,特别是其有效的抗炎作用。这一发现为开发一种新的人类健康治疗方案开辟了一条充满希望的道路,为医学的未来注入了希望。
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来源期刊
Molecular Nutrition & Food Research
Molecular Nutrition & Food Research 工程技术-食品科技
CiteScore
8.70
自引率
1.90%
发文量
250
审稿时长
1.7 months
期刊介绍: Molecular Nutrition & Food Research is a primary research journal devoted to health, safety and all aspects of molecular nutrition such as nutritional biochemistry, nutrigenomics and metabolomics aiming to link the information arising from related disciplines: Bioactivity: Nutritional and medical effects of food constituents including bioavailability and kinetics. Immunology: Understanding the interactions of food and the immune system. Microbiology: Food spoilage, food pathogens, chemical and physical approaches of fermented foods and novel microbial processes. Chemistry: Isolation and analysis of bioactive food ingredients while considering environmental aspects.
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