Magnetic solid phase microextraction of cadmium in water and food samples on Escherichia coli immobilized to magnetic conductive carbon black (Vulcan XC-72) prior to its determination by flame atomic absorption spectrometry

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-06-27 DOI:10.1016/j.foodchem.2025.145267

Mohammed Majid, Demirhan Citak, Mustafa Tuzen

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引用次数: 0

Abstract

Magnetic solid phase microextraction method (MSPME) is suggested due to simpler, faster, and utilizes less solvent than standard extraction methods. Non-active bacteria (Escherichia coli) were used as biosorbents and immobilized on support magnetic conductive carbon black (Vulcan XC-72) to preconcentrate cadmium. This new magnetic biosorbent composite was characterized using FTIR, SEM-EDX, and BET. Central composite design (CCD) and Plackett-Burman design (PBD) were applied to evaluate pH, biosorbent amount, sample volume, and vortex time relationships to achieve an optimum response. The matrix effects of certain cations and anions on analyte ions were examined. Limits of detection (LOD) and quantification (LOQ) were 0.6 and 2.0 μg L⁻¹, respectively. Preconcentration factor (PF) and enhancement factor (EF) were 100 and 88, and the adsorbent capacity was 90.3 mg g⁻¹. Intraday and interday RSD were 1.88 and 1.98 %. The calibration curve was linear from 2.0 to 40 μg L⁻¹. Method accuracy was verified by analyzing certified reference materials. The optimized approach achieved high efficiency in recovering cadmium in water, food, and soft drink samples, showing an affordable and fast technique for analyzing food directly at the source, providing valuable support for safeguarding public health and monitoring environmental safety.

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磁性固相微萃取水和食品样品中的镉，在固定化大肠杆菌上固定化导电炭黑（Vulcan XC-72），然后用火焰原子吸收光谱法测定

磁固相微萃取法（MSPME）比传统的萃取方法简单、快速、溶剂用量少。采用非活性菌（大肠杆菌）作为生物吸附剂，固定化载体导电炭黑（Vulcan XC-72）对镉进行预富集。利用FTIR、SEM-EDX和BET对这种新型磁性生物吸附剂进行了表征。采用中心复合设计（CCD）和Plackett-Burman设计（PBD）对pH、生物吸附剂用量、样品体积和涡旋时间关系进行评价，以获得最佳响应。考察了某些阳离子和阴离子对分析物离子的基质效应。检出限（LOD）为0.6，定量限（LOQ）为2.0 μg L−1。预富集因子（PF）和增强因子（EF）分别为100和88，吸附量为90.3 mg g−1。日内和日内RSD分别为1.88和1.98 %。标定曲线在2.0 ~ 40 μg L−1范围内呈线性。通过对标准物质的分析，验证了方法的准确性。优化后的方法在水、食品和软饮料样品中实现了镉的高效回收，展示了一种经济、快速的直接从源头分析食品的技术，为保障公众健康和监测环境安全提供了有价值的支持。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.