Recovery from Resistance Exercise with or Without Blood Flow Restriction Results in an Early Biphasic Pattern of Change in Albumin Cys34 Oxidation in Untrained Males.
Zi Xiang Lim, Jackson Peos, Stefan Ostojic, Peter G Arthur, Paul A Fournier
{"title":"Recovery from Resistance Exercise with or Without Blood Flow Restriction Results in an Early Biphasic Pattern of Change in Albumin Cys34 Oxidation in Untrained Males.","authors":"Zi Xiang Lim, Jackson Peos, Stefan Ostojic, Peter G Arthur, Paul A Fournier","doi":"10.3390/antiox14060667","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Oxidative stress contributes to the activation of muscle protein synthesis after high-intensity resistance exercise (HIRE) or low-intensity resistance exercise combined with blood flow restriction (LIBFR), but it is unclear if this oxidative stress response post-exercise is monophasic or multiphasic. We aimed to answer this question using albumin Cys34 oxidation as an oxidative stress marker.</p><p><strong>Methods: </strong>Seven untrained individuals completed HIRE and LIBFR on separate days. Albumin Cys34 oxidation (total and reversibly and irreversibly oxidized fractions), muscle oxygenation, oxygen consumption (V˙O2), lactate, and heart rate (HR) were measured before and up to 5 h post-exercise.</p><p><strong>Results: </strong>Both HIRE and LIBFR induced a biphasic increase in total oxidized albumin Cys34, with a transient peak in irreversibly oxidized albumin Cys34 immediately post-exercise (<i>p</i> < 0.001) before a delayed sustained increase in reversibly oxidized albumin Cys34, which peaked at 90-120 min and lasted ≥5 h post-exercise (<i>p</i> < 0.05). Muscle oxygenation decreased immediately post-exercise (<i>p</i> < 0.001) before rising above baseline (<i>p</i> < 0.05). V˙O2, HR, and blood lactate peaked post-exercise (<i>p</i> < 0.001) and returned to baseline within 15-90 min. Irreversibly oxidized albumin Cys34 was positively correlated with lactate and V˙O2 post-exercise (<i>p</i> < 0.001).</p><p><strong>Conclusion: </strong>Here, we show that resistance exercise, with or without blood flow restriction, results in an early biphasic oxidative stress response after exercise.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 6","pages":""},"PeriodicalIF":6.0000,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189344/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antioxidants","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/antiox14060667","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Oxidative stress contributes to the activation of muscle protein synthesis after high-intensity resistance exercise (HIRE) or low-intensity resistance exercise combined with blood flow restriction (LIBFR), but it is unclear if this oxidative stress response post-exercise is monophasic or multiphasic. We aimed to answer this question using albumin Cys34 oxidation as an oxidative stress marker.
Methods: Seven untrained individuals completed HIRE and LIBFR on separate days. Albumin Cys34 oxidation (total and reversibly and irreversibly oxidized fractions), muscle oxygenation, oxygen consumption (V˙O2), lactate, and heart rate (HR) were measured before and up to 5 h post-exercise.
Results: Both HIRE and LIBFR induced a biphasic increase in total oxidized albumin Cys34, with a transient peak in irreversibly oxidized albumin Cys34 immediately post-exercise (p < 0.001) before a delayed sustained increase in reversibly oxidized albumin Cys34, which peaked at 90-120 min and lasted ≥5 h post-exercise (p < 0.05). Muscle oxygenation decreased immediately post-exercise (p < 0.001) before rising above baseline (p < 0.05). V˙O2, HR, and blood lactate peaked post-exercise (p < 0.001) and returned to baseline within 15-90 min. Irreversibly oxidized albumin Cys34 was positively correlated with lactate and V˙O2 post-exercise (p < 0.001).
Conclusion: Here, we show that resistance exercise, with or without blood flow restriction, results in an early biphasic oxidative stress response after exercise.
AntioxidantsBiochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍:
Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.