Synergistic Effect of TiO2-Nanoparticles and Plant Growth-Promoting Microorganisms on the Physiological Parameters and Antioxidant Responses of Capsicum annum Cultivars.
Atiya Bhatti, Araceli Sanchez-Martinez, Gildardo Sanchez-Ante, Daniel A Jacobo-Velázquez, Joaquín Alejandro Qui-Zapata, Soheil S Mahmoud, Ghulam Mustafa Channa, Luis Marcelo Lozano, Jorge L Mejía-Méndez, Edgar R López-Mena, Diego E Navarro-López
{"title":"Synergistic Effect of TiO<sub>2</sub>-Nanoparticles and Plant Growth-Promoting Microorganisms on the Physiological Parameters and Antioxidant Responses of <i>Capsicum annum</i> Cultivars.","authors":"Atiya Bhatti, Araceli Sanchez-Martinez, Gildardo Sanchez-Ante, Daniel A Jacobo-Velázquez, Joaquín Alejandro Qui-Zapata, Soheil S Mahmoud, Ghulam Mustafa Channa, Luis Marcelo Lozano, Jorge L Mejía-Méndez, Edgar R López-Mena, Diego E Navarro-López","doi":"10.3390/antiox14060707","DOIUrl":null,"url":null,"abstract":"<p><p>Titanium dioxide nanoparticles (TiO<sub>2</sub>-NPs) were synthesized using the molten salt method and systematically characterized. TiO<sub>2</sub>-NPs were evaluated for their capacity to promote the growth of <i>Capsicum annuum</i> cultivars together with the plant growth-promoting microorganisms (PGPMs) <i>Bacillus thuringiensis</i> (Bt) and <i>Trichoderma harzianum</i> (Th). The variables analyzed included physiological parameters and antioxidant responses. The capacity of TiO<sub>2</sub>-NPs to scavenge free radicals was also investigated, along with their biocompatibility, using <i>Artemia salina</i> as an in vivo model. The results demonstrated that TiO<sub>2</sub>-NPs exhibited a nanocuboid-type morphology, negative surface charge, and small surface area. It was noted that TiO<sub>2</sub>-NPs enhanced the CFU and spore production of Bt (1.56-2.92 × 10<sup>8</sup> CFU/mL) and Th (2.50-3.90 × 10<sup>8</sup> spores/mL), respectively. It was observed that TiO<sub>2</sub>-NPs could scavenge DPPH, ABTS, and H<sub>2</sub>O<sub>2</sub> radicals (IC<sub>50</sub> 48.66-109.94 μg/mL), while not compromising the viability of <i>A. salina</i> at 50-300 μg/mL. TiO<sub>2</sub>-NPs were determined to enhance the root length and fresh and dry weights of chili peppers. Similarly, TiO<sub>2</sub>-NPs in synergy with Bt and Th increased the activity of β-1,3-Glucanase (2.45 nkat/g FW) and peroxidase (69.90 UA/g FW) enzyme activity, and increased the TPC (29.50 GA/g FW). The synergy of TiO<sub>2</sub>-NPs with the PGPMs consortium also upregulated the total chlorophyll content: 210.8 ± 11.4 mg/mg FW. The evidence from this study unveils the beneficial application of TiO<sub>2</sub>-NPs with Bt and Th as an efficient approach to promote the physiology and antioxidant responses of chili peppers.</p>","PeriodicalId":7984,"journal":{"name":"Antioxidants","volume":"14 6","pages":""},"PeriodicalIF":6.0000,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189501/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antioxidants","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/antiox14060707","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Titanium dioxide nanoparticles (TiO2-NPs) were synthesized using the molten salt method and systematically characterized. TiO2-NPs were evaluated for their capacity to promote the growth of Capsicum annuum cultivars together with the plant growth-promoting microorganisms (PGPMs) Bacillus thuringiensis (Bt) and Trichoderma harzianum (Th). The variables analyzed included physiological parameters and antioxidant responses. The capacity of TiO2-NPs to scavenge free radicals was also investigated, along with their biocompatibility, using Artemia salina as an in vivo model. The results demonstrated that TiO2-NPs exhibited a nanocuboid-type morphology, negative surface charge, and small surface area. It was noted that TiO2-NPs enhanced the CFU and spore production of Bt (1.56-2.92 × 108 CFU/mL) and Th (2.50-3.90 × 108 spores/mL), respectively. It was observed that TiO2-NPs could scavenge DPPH, ABTS, and H2O2 radicals (IC50 48.66-109.94 μg/mL), while not compromising the viability of A. salina at 50-300 μg/mL. TiO2-NPs were determined to enhance the root length and fresh and dry weights of chili peppers. Similarly, TiO2-NPs in synergy with Bt and Th increased the activity of β-1,3-Glucanase (2.45 nkat/g FW) and peroxidase (69.90 UA/g FW) enzyme activity, and increased the TPC (29.50 GA/g FW). The synergy of TiO2-NPs with the PGPMs consortium also upregulated the total chlorophyll content: 210.8 ± 11.4 mg/mg FW. The evidence from this study unveils the beneficial application of TiO2-NPs with Bt and Th as an efficient approach to promote the physiology and antioxidant responses of chili peppers.
AntioxidantsBiochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍:
Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.