Synergistic Effect of TiO2-Nanoparticles and Plant Growth-Promoting Microorganisms on the Physiological Parameters and Antioxidant Responses of Capsicum annum Cultivars.

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Atiya Bhatti, Araceli Sanchez-Martinez, Gildardo Sanchez-Ante, Daniel A Jacobo-Velázquez, Joaquín Alejandro Qui-Zapata, Soheil S Mahmoud, Ghulam Mustafa Channa, Luis Marcelo Lozano, Jorge L Mejía-Méndez, Edgar R López-Mena, Diego E Navarro-López
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引用次数: 0

Abstract

Titanium dioxide nanoparticles (TiO2-NPs) were synthesized using the molten salt method and systematically characterized. TiO2-NPs were evaluated for their capacity to promote the growth of Capsicum annuum cultivars together with the plant growth-promoting microorganisms (PGPMs) Bacillus thuringiensis (Bt) and Trichoderma harzianum (Th). The variables analyzed included physiological parameters and antioxidant responses. The capacity of TiO2-NPs to scavenge free radicals was also investigated, along with their biocompatibility, using Artemia salina as an in vivo model. The results demonstrated that TiO2-NPs exhibited a nanocuboid-type morphology, negative surface charge, and small surface area. It was noted that TiO2-NPs enhanced the CFU and spore production of Bt (1.56-2.92 × 108 CFU/mL) and Th (2.50-3.90 × 108 spores/mL), respectively. It was observed that TiO2-NPs could scavenge DPPH, ABTS, and H2O2 radicals (IC50 48.66-109.94 μg/mL), while not compromising the viability of A. salina at 50-300 μg/mL. TiO2-NPs were determined to enhance the root length and fresh and dry weights of chili peppers. Similarly, TiO2-NPs in synergy with Bt and Th increased the activity of β-1,3-Glucanase (2.45 nkat/g FW) and peroxidase (69.90 UA/g FW) enzyme activity, and increased the TPC (29.50 GA/g FW). The synergy of TiO2-NPs with the PGPMs consortium also upregulated the total chlorophyll content: 210.8 ± 11.4 mg/mg FW. The evidence from this study unveils the beneficial application of TiO2-NPs with Bt and Th as an efficient approach to promote the physiology and antioxidant responses of chili peppers.

纳米tio2与植物促生微生物对辣椒生理参数及抗氧化反应的协同效应
采用熔盐法制备了二氧化钛纳米颗粒(TiO2-NPs),并对其进行了系统表征。研究了TiO2-NPs与植物生长促进微生物苏云金芽孢杆菌(Bacillus thuringiensis, Bt)和哈兹木霉(Trichoderma harzianum, Th)共同促进辣椒品种生长的能力。分析的变量包括生理参数和抗氧化反应。以盐渍蒿(Artemia salina)为体内模型,研究了TiO2-NPs清除自由基的能力及其生物相容性。结果表明,TiO2-NPs具有纳米立方体形态,表面带负电荷,表面积小。结果表明,TiO2-NPs可提高Bt (1.56 ~ 2.92 × 108 CFU/mL)和Th (2.50 ~ 3.90 × 108孢子/mL)的CFU和孢子产量。结果表明,TiO2-NPs可清除DPPH、ABTS和H2O2自由基(IC50为48.66 ~ 109.94 μg/mL),且在50 ~ 300 μg/mL浓度下不影响盐藻的活性。测定了TiO2-NPs对辣椒根长、鲜重和干重的影响。同样,TiO2-NPs与Bt和Th协同作用提高了β-1,3-葡聚糖酶(2.45 nkat/g FW)和过氧化物酶(69.90 UA/g FW)活性,提高了TPC (29.50 GA/g FW)。TiO2-NPs与PGPMs联合体的协同作用也上调了总叶绿素含量:210.8±11.4 mg/mg FW。本研究的证据揭示了含Bt和Th的TiO2-NPs作为促进辣椒生理和抗氧化反应的有效途径的有益应用。
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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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