Yang Cao, Fei Hao, Jingpeng Li, Bolun Zhang, Zeming Li, Tiantian Liu, Yan Gao, Xuguang Niu, Xiaohu Liu, Hui Zhang, Lijuan Yang
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引用次数: 0
Abstract
Alkaline stress (AS) is one of the major threats that severely affects rice growth and grain yield. However, the differences in the damage caused by the main components of soda saline-alkali land, sodium carbonate (Na2CO3), and sodium bicarbonate (NaHCO3) to rice seedlings are still unclear. This study explored the effects of different carbonate stresses (Na2CO3 and NaHCO3) on rice seedling growth, root damage, physiological responses, and molecular changes. By administering equivalent concentrations of sodium ions through these different carbonate treatments, we observed that both stresses significantly inhibited rice growth. However, the inhibitory effect was more pronounced under the Na2CO3 treatment. Compared with the NaHCO3 treatment, Na2CO3 stress caused more severe damage to root cell membranes and led to a substantial decline in root vigor. Moreover, the contents of reactive oxygen species (ROS) and malondialdehyde (MDA) were markedly increased, indicating that Na2CO3 induces more severe oxidative damage. Transcriptomic and metabolomic analyses revealed a greater number of differentially expressed genes (DEGs) and differentially expressed metabolites (DEMs) in the Na2CO3 treatment group. The integrative analysis and validation demonstrated that pathways related to auxin, ascorbate, flavonoids, and glutathione metabolism were particularly enriched under Na2CO3 stress. These findings suggest that Na2CO3 stress may interfere with auxin signaling pathways and exerts a more profound impact on endogenous antioxidant systems, affecting rice growth at multiple levels. In summary, this research highlights the differential impacts of Na2CO3 and Na2CO3 stresses on rice seedling growth, physiology, and molecular processes, particularly oxidative damage and antioxidant responses. The insights gained provide a valuable theoretical foundation for enhancing rice alkali tolerance and developing strategies for the rational cultivation of rice in saline-alkaline soils.
AntioxidantsBiochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍:
Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.