Evaluation of Bioactive Properties of Ultrasound-Assisted Extracts from Prokupac Grape Skins for Functional Foods.

IF 6.6 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Antioxidants Pub Date : 2025-06-15 DOI:10.3390/antiox14060733

Edina Avdović, Dušan Dimić, Đura Nakarada, Dušica Simijonović, Sandra Jovičić Milić, Katarina Marković, Mirjana Grujović, Marko Antonijević, Andrija Ćirić, Dejan Milenković, Zoran Marković

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引用次数: 0

Abstract

The phenolic compounds present in wine industry by-products are a valuable source of biologically active ingredients that could be used in the development of functional foods. This manuscript investigates the potential of ultrasound-assisted extracts from Prokupac grape skins-a wine industry by-product-as functional food ingredients. Four extracts were prepared using different solvents and evaluated for their antioxidant, anti-inflammatory, and antimicrobial properties. Antioxidant activity was assessed through DPPH, ABTS, and FRAP assays, as well as EPR spectroscopy. Phenolic composition was determined via HPLC analysis, and anti-inflammatory potential was evaluated using a lipoxygenase inhibition assay. Results indicated that the extracts PSE3 (ethyl acetate) and PSE0 (direct extraction with 50% ethanol) exhibited superior antioxidant and anti-inflammatory activities, which can be attributed to their high polyphenolic content. Additionally, the extracts demonstrated antimicrobial effects against the tested microorganisms. These findings suggest that Prokupac grape skin extracts, particularly PSE3 and PSE0, could be valuable additions to functional foods, offering health benefits through their bioactive properties.

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功能食品用葡萄皮超声萃取物的生物活性评价。

葡萄酒工业副产品中的酚类化合物是生物活性成分的宝贵来源，可用于功能性食品的开发。本文研究了超声辅助提取普罗库帕克葡萄皮作为功能性食品成分的潜力，普罗库帕克葡萄皮是葡萄酒工业的副产品。采用不同的溶剂制备了四种提取物，并对其抗氧化、抗炎和抗菌性能进行了评价。通过DPPH、ABTS和FRAP测定以及EPR光谱法评估其抗氧化活性。用高效液相色谱法测定酚类成分，用脂氧合酶抑制法评价抗炎潜力。结果表明，提取物PSE3（乙酸乙酯）和PSE0（50%乙醇直接提取）具有较强的抗氧化和抗炎活性，这可能与其高多酚含量有关。此外，提取物对被测微生物具有抗菌作用。这些发现表明，Prokupac葡萄皮提取物，特别是PSE3和PSE0，可能是功能性食品中有价值的添加物，通过其生物活性特性提供健康益处。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology

CiteScore

10.60

自引率

11.40%

发文量

2123

审稿时长

16.3 days

期刊介绍： Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.