A Hydrolyzed Soybean Protein Enhances Oxidative Stress Resistance in C. elegans and Modulates Gut-Immune Axis in BALB/c Mice.

IF 6 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Jun Liu, Yansheng Zhao, Fei Leng, Xiang Xiao, Weibo Jiang, Shuntang Guo
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引用次数: 0

Abstract

Soy protein isolate (SPI) is a high-purity protein from defatted soybeans, providing emulsifying and gelling functions for plant-based foods and supplements. Hydrolysis can facilitate the production of bioactive small-molecule proteins or peptides with potential functional applications. In this study, 20% hydrolyzed soy protein (20% HSP) was prepared from SPI, and the effects of 20% HSP and SPI on alleviating oxidative stress in Caenorhabditis elegans (C. elegans) and regulating immune-gut microbiota in cyclophosphamide (CTX)-induced immunocompromised BALB/c mice were investigated. In C. elegans, both SPI and 20% HSP (300 μg/mL) enhanced locomotive activities, including body bending and head thrashing, and improved oxidative stress resistance under high glucose conditions. This improvement was mediated by increased antioxidant enzyme activities (SOD, CAT, and GSH-Px), while malondialdehyde (MDA) content was reduced by 60.15% and 82.28%, respectively. Both of them can also significantly extend the lifespan of normal C. elegans and paraquat-induced oxidative stress models by inhibiting lipofuscin accumulation. This effect was mediated through upregulation of daf-16 and suppression of daf-2 and akt-1 expression. In immunocompromised mice, 20% HSP alleviated CTX-induced immune dysfunction by increasing peripheral white blood cells and lymphocytes, attenuating thymic atrophy, and reducing hepatic oxidative stress via MDA inhibition. Gut microbiota analysis revealed that 20% HSP restored microbial balance by suppressing Escherichia-Shigella and enriching beneficial genera, like Psychrobacter. These findings highlight 20% HSP and SPI's conserved anti-aging mechanisms via daf-16 activation in C. elegans and immune-gut modulation in mice, positioning them as plant-derived nutraceuticals targeting oxidative stress and immune dysregulation.

一种水解大豆蛋白增强秀丽隐杆线虫的氧化应激抗性并调节BALB/c小鼠的肠道免疫轴
大豆分离蛋白(SPI)是从脱脂大豆中提取的高纯度蛋白质,为植物性食品和补充剂提供乳化和胶凝功能。水解可以促进生产具有潜在功能应用的生物活性小分子蛋白质或肽。本研究以大豆分离蛋白为原料制备20%水解大豆蛋白(20% HSP),研究20%水解大豆蛋白和大豆分离蛋白对秀丽隐杆线虫(c . elegans)氧化应激和调节环磷酰胺(CTX)诱导的免疫功能受损BALB/c小鼠免疫肠道微生物群的影响。在高糖条件下,SPI和20% HSP (300 μg/mL)均能增强秀丽隐杆线虫的运动活性,包括身体弯曲和头部捶打,并提高其抗氧化应激能力。这主要是由于抗氧化酶(SOD、CAT和GSH-Px)活性增加,丙二醛(MDA)含量分别降低了60.15%和82.28%。它们还可以通过抑制脂褐素积累,显著延长正常秀丽隐杆线虫和百草枯诱导的氧化应激模型的寿命。这种作用是通过上调daf-16和抑制daf-2和akt-1表达介导的。在免疫功能低下的小鼠中,20%热休克蛋白通过增加外周血白细胞和淋巴细胞、减轻胸腺萎缩和通过MDA抑制降低肝脏氧化应激来减轻ctx诱导的免疫功能障碍。肠道菌群分析显示,20%热休克蛋白通过抑制志贺氏杆菌和丰富有益菌(如冷杆菌)来恢复微生物平衡。这些发现强调了20%热休克蛋白和大豆大豆蛋白通过秀丽隐杆线虫的daf-16激活和小鼠的免疫肠道调节而保守的抗衰老机制,将它们定位为针对氧化应激和免疫失调的植物源性营养保健品。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Antioxidants
Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
10.60
自引率
11.40%
发文量
2123
审稿时长
16.3 days
期刊介绍: Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.
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