Brassinolide-induced resistance enhances antioxidant defense and metabolic pathways against anthracnose in Camellia sinensis ‘Fuding Dabaicha’

IF 3.9 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-06-27 DOI:10.1016/j.scienta.2025.114258

Wei Liu , Xiyong Guo , Qiang Chen , Lijuan Fu , Rifeng Wei , Muhammad Moaaz Ali , Muhammad Suliman Shah

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Abstract

This study investigated the effect of exogenous brassinolide (BR) treatment on managing anthracnose in Fuding Dabaicha tea (FDT) plants following inoculation with Colletotrichum gloeosporioides (Cg). By analyzing changes in antioxidant enzyme activities, transcriptomic profiles, and metabolomic alterations, we evaluated the induced resistance of FDT to anthracnose after BR treatment and the associated physiological and biochemical responses. The results demonstrated that BR pretreatment before Cg inoculation effectively delayed disease progression and enhanced the activities of peroxidase (POD) and glutathione-S-transferase (GST). Transcriptomic analysis revealed that the comparison between Cg-inoculated control and BR-treated plants (CK-Cg vs. BR-Cg) showed significant enrichment in glutathione metabolism, plant hormone signaling, and phenylpropanoid biosynthesis pathways. Differentially expressed genes (DEGs) were particularly associated with phenylalanine metabolism and flavonoid biosynthesis. Metabolomic analysis further indicated that BR treatment may strengthen plant defense mechanisms by promoting the synthesis of flavonoids, flavonols, and diterpenoids. KEGG enrichment analysis of differentially expressed genes and metabolites highlighted significant enrichment in glutathione metabolism, flavonoid biosynthesis, phenylalanine metabolism, and various secondary metabolite biosynthesis pathways in both CK vs BR and CK-Cg vs BR-Cg comparisons.

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油菜素内酯诱导的抗性增强了福鼎大白茶抗炭疽病的抗氧化防御和代谢途径

本研究研究了外源油菜素内酯（BR）处理对福鼎大白茶（FDT）接种炭疽菌（Colletotrichum gloeosporioides, Cg）后防治炭疽病的影响。通过分析抗氧化酶活性、转录组学和代谢组学的变化，我们评估了BR处理后FDT对炭疽病的诱导抗性以及相关的生理生化反应。结果表明，接种Cg前BR预处理可有效延缓疾病进展，提高过氧化物酶（POD）和谷胱甘肽- s转移酶（GST）活性。转录组学分析显示，接种cg的对照和br处理的植株（CK-Cg vs BR-Cg）在谷胱甘肽代谢、植物激素信号传导和苯丙素生物合成途径上显著富集。差异表达基因（DEGs）与苯丙氨酸代谢和类黄酮生物合成密切相关。代谢组学分析进一步表明，BR处理可能通过促进黄酮类化合物、黄酮醇和二萜的合成来增强植物的防御机制。差异表达基因和代谢物的KEGG富集分析显示，在CK与BR和CK- cg与BR- cg比较中，谷胱甘肽代谢、类黄酮生物合成、苯丙氨酸代谢和各种次级代谢物生物合成途径中都有显著富集。

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.