The Prevalence of Antimicrobial Resistance Genes in the Environments of Small Ruminant Farms from Central Portugal.

IF 4.3 2区 医学 Q1 INFECTIOUS DISEASES
Jaqueline T Bento, Sara Gomes-Gonçalves, Rita Cruz, Fernando Esteves, Alexandra Lameira Baptista, Maria Aires Pereira, Pedro Caseiro, Pedro Carreira, Luís Figueira, João R Mesquita, Adriano A Bordalo, Ana Machado
{"title":"The Prevalence of Antimicrobial Resistance Genes in the Environments of Small Ruminant Farms from Central Portugal.","authors":"Jaqueline T Bento, Sara Gomes-Gonçalves, Rita Cruz, Fernando Esteves, Alexandra Lameira Baptista, Maria Aires Pereira, Pedro Caseiro, Pedro Carreira, Luís Figueira, João R Mesquita, Adriano A Bordalo, Ana Machado","doi":"10.3390/antibiotics14060576","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Antimicrobial resistance is a pressing global concern affecting both human and animal health, with environment playing a key role in the dissemination of resistance determinants. This study aimed to investigate the presence of antimicrobial resistance genes (ARGs) associated with tetracyclines, β-lactams, macrolides, and sulfonamides in environmental matrices collected from 65 sheep and goat farms in central Portugal.</p><p><strong>Methods: </strong>Environmental samples, including water, soil, pasture, and bedding, were analyzed through qPCR for the detection of clinically relevant ARGs.</p><p><strong>Results: </strong>ARGs were detected in 83% of the samples, with over half exhibiting genes from three or more antibiotic classes, suggesting potential multidrug resistance. β-lactamase genes were the most prevalent, followed by those conferring resistance to tetracycline and sulfonamide resistance, while macrolide resistance genes were least frequent. The distribution of ARGs varied by farm type, host species, and municipality.</p><p><strong>Conclusions: </strong>These findings suggest that small ruminant farms serve as important reservoirs for ARGs. The results underscore the need for systematic surveillance and further research into the ecological and genetic factors driving ARG persistence and dissemination in extensive livestock systems, including proper waste management strategies to limit the spread and persistence of antibiotic resistance and mitigate broader public health risks.</p>","PeriodicalId":54246,"journal":{"name":"Antibiotics-Basel","volume":"14 6","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12189902/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Antibiotics-Basel","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3390/antibiotics14060576","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Antimicrobial resistance is a pressing global concern affecting both human and animal health, with environment playing a key role in the dissemination of resistance determinants. This study aimed to investigate the presence of antimicrobial resistance genes (ARGs) associated with tetracyclines, β-lactams, macrolides, and sulfonamides in environmental matrices collected from 65 sheep and goat farms in central Portugal.

Methods: Environmental samples, including water, soil, pasture, and bedding, were analyzed through qPCR for the detection of clinically relevant ARGs.

Results: ARGs were detected in 83% of the samples, with over half exhibiting genes from three or more antibiotic classes, suggesting potential multidrug resistance. β-lactamase genes were the most prevalent, followed by those conferring resistance to tetracycline and sulfonamide resistance, while macrolide resistance genes were least frequent. The distribution of ARGs varied by farm type, host species, and municipality.

Conclusions: These findings suggest that small ruminant farms serve as important reservoirs for ARGs. The results underscore the need for systematic surveillance and further research into the ecological and genetic factors driving ARG persistence and dissemination in extensive livestock systems, including proper waste management strategies to limit the spread and persistence of antibiotic resistance and mitigate broader public health risks.

葡萄牙中部小型反刍动物养殖场环境中抗微生物药物耐药性基因的流行。
背景:抗菌素耐药性是影响人类和动物健康的一个紧迫的全球问题,环境在耐药性决定因素的传播中起着关键作用。本研究旨在调查从葡萄牙中部65个绵羊和山羊农场收集的环境基质中与四环素类、β-内酰胺类、大环内酯类和磺胺类相关的抗微生物药物耐药性基因(ARGs)的存在。方法:采用qPCR方法对水、土壤、牧草、床上用品等环境样品进行分析,检测临床相关ARGs。结果:在83%的样本中检测到ARGs,其中一半以上显示来自三种或更多抗生素类别的基因,表明可能存在多药耐药。β-内酰胺酶基因最为普遍,其次是四环素和磺胺耐药基因,而大环内酯类耐药基因最少。ARGs的分布因农场类型、宿主种类和城市而异。结论:这些发现表明,小型反刍动物养殖场是ARGs的重要储存库。这些结果强调需要系统监测和进一步研究驱动ARG在广泛的牲畜系统中持续存在和传播的生态和遗传因素,包括适当的废物管理战略,以限制抗生素耐药性的传播和持续存在,并减轻更广泛的公共卫生风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Antibiotics-Basel
Antibiotics-Basel Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics
CiteScore
7.30
自引率
14.60%
发文量
1547
审稿时长
11 weeks
期刊介绍: Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信