Ability of Linezolid to Combat Staphylococcus aureus and Pseudomonas aeruginosa Isolated from Polymicrobial Wound Infections.

IF 4.3 2区 医学 Q1 INFECTIOUS DISEASES
Samar A Ahmed, Vy T Luu, Teresa C Oyono Nsuga, Steven E Burgos, Eugene Kreys, Jered Arquiette, Justin R Lenhard
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引用次数: 0

Abstract

Background/Objectives: The optimal therapy for polymicrobial wound infections is poorly defined. We sought to characterize the ability of linezolid to combat mixed cultures of Staphylococcus aureus and Pseudomonas aeruginosa. Methods: The antistaphylococcal activity of linezolid was assessed in 24-h time-killing experiments that used S. aureus and P. aeruginosa isolated from polymicrobial wound infections. Clindamycin was also evaluated as a comparator. A Hill-type mathematical model was used to assess the maximum killing of S. aureus (Emax). The ability of linezolid to potentiate the activity of host defense peptides against P. aeruginosa was evaluated using LL-37. Results: In the presence of P. aeruginosa, the Emax of linezolid decreased in 5/9 co-culture experiments and increased in 4/9 co-culture experiments in comparison to linezolid against S. aureus alone. The potency of linezolid was not significantly impacted by the presence of P. aeruginosa. In comparison, the maximal S. aureus killing achieved by clindamycin decreased in eight out of nine experiments, and somewhat paradoxically, the potency increased in nine out of nine experiments. In the host defense peptide assay, the supratherapeutic linezolid concentration of 64 mg/L did not significantly enhance the killing of the LL-37 peptides (p ≥ 0.121), but the concentration of linezolid was significantly associated with the killing of one of three P. aeruginosa isolates (p = 0.005). Conclusions: P. aeruginosa had a minimal impact on the antistaphylococcal activity of linezolid in comparison to clindamycin. Linezolid did not exert a consistent ability to enhance the antipseudomonal activity of host defense peptides. These data may help inform antimicrobial selection during polymicrobial wound infections.

利奈唑胺对多微生物伤口感染中金黄色葡萄球菌和铜绿假单胞菌的抑制作用。
背景/目的:多微生物伤口感染的最佳治疗方法尚不明确。我们试图表征利奈唑胺对抗金黄色葡萄球菌和铜绿假单胞菌混合培养的能力。方法:采用多微生物创面感染分离的金黄色葡萄球菌和铜绿假单胞菌进行24h时间杀伤实验,评价利奈唑胺的抗葡萄球菌活性。克林霉素也被评价为比较物。采用hill型数学模型对金黄色葡萄球菌(S. aureus, Emax)的最大杀伤效果进行了评价。利用LL-37检测了利奈唑胺增强宿主防御肽对铜绿假单胞菌活性的能力。结果:在铜绿假单胞菌存在时,与利奈唑胺单独抗金黄色葡萄球菌相比,5/9共培养实验中利奈唑胺的Emax降低,4/9共培养实验中利奈唑胺的Emax升高。利奈唑胺的效力不受铜绿假单胞菌存在的显著影响。相比之下,克林霉素对金黄色葡萄球菌的最大杀伤作用在9个实验中有8个实验中下降,而有些矛盾的是,在9个实验中有9个实验中效力增加。在宿主防御肽实验中,64 mg/L的利奈唑胺对LL-37肽的杀伤作用不显著(p≥0.121),但对3株铜绿假单胞菌中1株的杀伤作用显著(p = 0.005)。结论:与克林霉素相比,铜绿假单胞菌对利奈唑胺的抗葡萄球菌活性影响最小。利奈唑胺对增强宿主防御肽的抗假单胞菌活性没有一致的作用。这些数据可能有助于在多微生物伤口感染期间选择抗微生物药物。
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来源期刊
Antibiotics-Basel
Antibiotics-Basel Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics
CiteScore
7.30
自引率
14.60%
发文量
1547
审稿时长
11 weeks
期刊介绍: Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.
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